ASGPR1 Antibodies

HAF005). A specific band was detected for ASGR1/ASGPR1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF005). A specific band was detected for ASGR1/ASGPR1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse liver tissue, human liver tissue (negative control), and HepG2 human hepatocellular carcinoma cell line (negative control). PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB27552) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for ASGR1/ASGPR1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB27551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB27552) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB27551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB27552) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

HAF017). A specific band was detected for ASGR1/ASGPR1 at approximately 40-46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for ASGR1/ASGPR1 at approximately 40-46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of mouse liver tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ASGR1/ASGPR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2755) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ASGR1/ASGPR1 at approximately 40-46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB0041, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB0041, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB43941, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and membranes in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and membranes in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB4394, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Albumin was also detected using Goat Anti-Human/Mouse Serum Albumin Antibody (Catalog # AF3329). Cells were co-stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Specific staining of ASGR1/ ASGPR1 was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Albumin was also detected using Goat Anti-Human/Mouse Serum Albumin Antibody (Catalog # AF3329). Cells were co-stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Specific staining of ASGR1/ ASGPR1 was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_thumb" />

DY990). Rat Anti-Mouse ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB27552) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Rat Anti-Mouse ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB27552) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Mouse ASGR1/ASGPR1 protein was serially diluted 2-fold and captured by Rat Anti-Mouse ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB27551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse ASGR1/ASGPR1 Monoclonal Antibody (Catalog # MAB27552) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />