Flow Cytometry: ASGPR1 Antibody (8D7) [NBP1-60150] - Detection of ASGPR1 in HepG2 cells. Red, black and blue line represent the isotype control, cells only, and an ASGPR1 antibody conjugated to FITC with a concentration ...read more
This antibody can be used in Flow Cytometry, ELISA, Immunofluorescence, Immunohistochemistry-Frozen and Western Blot. Western Blot: it is recommended to run under non-reducing conditions. The band size is approximately 42 kDa. Immunohistochemistry-Frozen: acetone fixation is recommended. ELISA: it is recommended to coat microtiter plates with antibody at 20ug/ml. Recommended controls: Positive: Rat hepatocytes; Negative: Rat kidney, pancreas, small intestine, and colon. Dilutions to be used depend on the detection system that is applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Read 1 Review rated 4 using NBP1-60150 in the following applications:
Use in Mouse reported in scientific literature (PMID:33554615).
Packaging, Storage & Formulations
Store at 4C.
0.2 um filtered solution in PBS and 0.1% BSA
0.02% Sodium Azide
Protein G purified
Alternate Names for ASGPR1 Antibody (8D7)
asialoglycoprotein receptor 1
C-type lectin domain family 4 member H1
Hepatic lectin H1
The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). Characterization of the ASGP receptor revealed its functional role in the binding, internalization and transport of a wide range of glycoproteins, which have exposed galactose or N-acetylgalactosamine residues, via the process of receptor-mediated endocytosis (RME). The ASGP receptor can bind a variety of important plasma proteins including transport proteins (i.e. transferrin), enzymes such as alkaline phosphatase, immunoglobulins including IgA, apoptotic hepatocytes, fibronectin and platelets. Additionally, the expression of the ASGP receptor has been clinically correlated to the level of hepatic function that is lost during liver diseases related to cancer, viral hepatitis, and cirrhosis. The ASGP receptor consists of major and minor subunits, which in the rat were identified as rat hepatic lectin (RHL) 1 and RHL 2/3, with molecular weights of respectively 42, 49 and 54 kDa. The selective binding (calcium and pH depended) and uptake of terminal galactosyl bearing proteins requires the formation of hetero-oligomers between these major and minor forms The total ASGP receptor population consisted of two functionally distinct receptor populations, designated State 1 and State 2, which were involved in the endocytosis and intracellular processing of ligands by different pathways. The monoclonal antibody 8D7 recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. The monoclonal antibody 8D7 is cross reactive with human ASGPR.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for ASGPR1 Antibody (NBP1-60150). (Showing 1 - 1 of 1 FAQs).
I am looking for ASGPR1 or any surface marker for hepatocytes reactive to mouse and monoclonal for flow cytometry application. I found NBP1-60150-0.025mg in your webpage. I was wondering if it would be a good fit for my application.
The antibody you are looking at (NBP1-60150-0.025mg) is validated and guaranteed for detection of mouse ASGPR1 in Flow. The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). The ASGP receptor consists of major and minor subunits, which in the rat were identified as rat hepatic lectin (RHL) 1 and RHL 2/3, with molecular weights of respectively 42, 49 and 54 kDa. This monoclonal antibody (clone 8D7) recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. The monoclonal antibody 8D7 is cross reactive with ASGPR from human, mouse and rat species.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.