ASGPR1 Antibody (8D7)

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IA, ICC/IF, IHC, IHC-Fr
Clone
8D7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

ASGPR1 Antibody (8D7) Summary

Immunogen
Crude rat liver membrane extracts
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ASGR1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunoassay
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
This antibody can be used in Flow Cytometry, Immunoassay, Immunofluorescence, Immunohistochemistry-Frozen and Western Blot. The typical starting working dilution is 1:10.
Publications
Read Publications using NBP1-60150.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28733363).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.2 um filtered solution in PBS and 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for ASGPR1 Antibody (8D7)

  • ASGPR 1
  • ASGP-R 1
  • ASGPR
  • ASGPR1
  • ASGR1
  • asialoglycoprotein receptor 1
  • CLEC4H1
  • CLEC4H1ASGPR1
  • C-type lectin domain family 4 member H1
  • Hepatic lectin H1
  • HL-1
  • MHL1
  • RHL1

Background

The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). Characterization of the ASGP receptor revealed its functional role in the binding, internalization and transport of a wide range of glycoproteins, which have exposed galactose or N-acetylgalactosamine residues, via the process of receptor-mediated endocytosis (RME). The ASGP receptor can bind a variety of important plasma proteins including transport proteins (i.e. transferrin), enzymes such as alkaline phosphatase, immunoglobulins including IgA, apoptotic hepatocytes, fibronectin and platelets. Additionally, the expression of the ASGP receptor has been clinically correlated to the level of hepatic function that is lost during liver diseases related to cancer, viral hepatitis, and cirrhosis. The ASGP receptor consists of major and minor subunits, which in the rat were identified as rat hepatic lectin (RHL) 1 and RHL 2/3, with molecular weights of respectively 42, 49 and 54 kDa. The selective binding (calcium and pH depended) and uptake of terminal galactosyl bearing proteins requires the formation of hetero-oligomers between these major and minor forms The total ASGP receptor population consisted of two functionally distinct receptor populations, designated State 1 and State 2, which were involved in the endocytosis and intracellular processing of ligands by different pathways. The monoclonal antibody 8D7 recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. The monoclonal antibody 8D7 is cross reactive with human ASGPR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASGPR1 Antibody (NBP1-60150)(8)

We have publications tested in 1 confirmed species: Mouse.


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Showing Publications 1 - 8 of 8.
Publications using NBP1-60150 Applications Species
Kiamehr M, Viiri LE, Vihervaara T et al. Lipidomic profiling of patient-specific induced pluripotent stem cell-derived hepatocyte-like cells Dis Model Mech 2017 Jul 21 [PMID: 28733363] (Mouse) Mouse
Zhou XL, Sun PN, Huang TH, Xie QD, Kang XJ, Liu LM. Effects of hepatitis B virus S protein on human sperm function. Hum Reprod;24(7):1575-83. 2009 Jul. [PMID: 19279032]
Keyel PA, Mishra SK, Roth R, Heuser JE, Watkins SC, Traub LM. A single common portal for clathrin-mediated endocytosis of distinct cargo governed by cargo-selective adaptors. Mol Biol Cell;17(10):4300-17. 2006 Oct. [PMID: 16870701]
Shimada M, Mizuno M, Uesu T, Nasu J, Okada H, Shimomura H, Yamamoto K, Tsuji T, Shiratori Y. A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit. Hepatol Res. 26(1):55-60. 2003 May. [PMID: 12787805]
Hirai M, Mizuno M, Morisue Y, Yoshioka M, Shimada M, Nasu J, Okada H, Shimomura H, Yamamoto K, Tsuji T. Development of syngeneic monoclonal anti-idiotype antibodies to mouse monoclonal anti-asialoglycoprotein receptor antibody. Source Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry, Japan. Acta Med Okayama. 56(3):135-9. 2002 Jun. [PMID: 12108584]
Hyodo I, Mizuno M, Yamada G, Tsuji T. Distribution of asialoglycoprotein receptor in human hepatocellular carcinoma. Liver;13(2):80-5. 1993 Apr. [PMID: 8389955]
Mizuno M, Yamada G, Sugiyama T, Vierling JM, Brown WR. Monoclonal antibodies identifying antigens on distinct domains of rat hepatocytes. Liver;7(5):251-9. 1987 Oct. [PMID: 3695813]
Mizuno M, Yamada G, Nagashima H. Development of a monoclonal antibody identifying an antigen which is segregated to the sinusoidal and lateral plasma membranes of rat hepatocytes. Gastroenterol Jpn;21(3):238-44. 1986 Jun. [PMID: 3525313]

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Bioinformatics

Gene Symbol ASGR1
Entrez