ASC/TMS1 Antibody [Unconjugated]

Western blot shows lysates of MCF-7 human breast cancer cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ASC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ASC at approximately 24 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and U937 human histiocytic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ASC at approximately 28 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ASC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3805) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Western blot shows lysates of MCF‑7 human breast cancer parental cell line and ASC knockout MCF‑7 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ASC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ASC at approximately 23 kDa (as indicated) in the parental MCF‑7 cell line, but is not detectable in knockoutMCF‑7 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, Simple Western, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ASC/TMS1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human ASC
  Leu112-Ser195
  Accession # Q9ULZ3
• Specificity: Detects human ASC in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PYCARD

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Knockout Validated

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ASC/TMS1 Antibody [Unconjugated]

• ASC
• ASCMGC10332
• CARD5
• CARD5TMS
• caspase recruitment domain protein 5
• Caspase recruitment domain-containing protein 5
• hASC
• PYCARD
• PYD and CARD domain containing
• PYD and CARD domain-containing protein
• Target of methylation-induced silencing 1
• TMS1
• TMS-1
• TMS1apoptosis-associated speck-like protein containing a CARD

Background

Apoptosis-associated speck-like protein containing a CARD (ASC) is also known as Target of Methylation-mediated Silencing (TMS-1) and PYD and CARD-domain containing protein (PYCARD). ASC is a p53 target protein that serves as an adaptor to the pro-apoptotic Bax molecule. The interaction of ASC with Bax promotes apoptosis in response to many apoptotic stimuli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASC/TMS1 Antibody (AF3805)(1)

Publication using AF3805

• CY Chang, HJ Shih, IT Huang, PS Tsai, KY Chen, CJ Huang Magnesium Sulfate Mitigates the Progression of Monocrotaline Pulmonary Hypertension in Rats Int J Mol Sci, 2019;20(18):. 2019 [PMID: 31540416] (IHC-P, Rat)

Bioinformatics

• Gene Symbol: PYCARD
• Uniprot:
  • Q9ULZ3()