ASC/TMS1 Antibody (OTI3E9) - Azide and BSA Free

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Western Blot: ASC/TMS1 Antibody (OTI3E9) - Azide and BSA Free [NBP2-71853] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASC/TMS1 (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunohistochemistry: ASC/TMS1 Antibody (OTI3E9) - Azide and BSA Free [NBP2-71853] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ASC/TMS1 mouse monoclonal antibody.
Western Blot: ASC/TMS1 Antibody (OTI3E9) - Azide and BSA Free [NBP2-71853] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-ASC/TMS1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; ...read more
Immunohistochemistry: ASC/TMS1 Antibody (OTI3E9) - Azide and BSA Free [NBP2-71853] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ASC/TMS1 mouse monoclonal antibody.
Immunohistochemistry: ASC/TMS1 Antibody (OTI3E9) - Azide and BSA Free [NBP2-71853] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ASC/TMS1 mouse monoclonal antibody.
Immunohistochemistry: ASC/TMS1 Antibody (OTI3E9) - Azide and BSA Free [NBP2-71853] - Staining of paraffin-embedded Human liver tissue using anti-ASC/TMS1 mouse monoclonal antibody.
Immunohistochemistry: ASC/TMS1 Antibody (OTI3E9) - Azide and BSA Free [NBP2-71853] - Staining of paraffin-embedded Human colon tissue using anti-ASC/TMS1 mouse monoclonal antibody.
CyTOF-ready: ASC/TMS1 Antibody (OTI3E9) - Azide and BSA Free [NBP2-71853] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-ASC/TMS1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
OTI3E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

ASC/TMS1 Antibody (OTI3E9) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human PYCARD (NP_037390) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PYCARD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • CyTOF-ready
Theoretical MW
21.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for ASC/TMS1 Antibody (OTI3E9) - Azide and BSA Free

  • apoptosis-associated speck-like protein containing a CARD
  • ASC
  • CARD5
  • caspase recruitment domain protein 5
  • Caspase recruitment domain-containing protein 5
  • hASC
  • PYCARD
  • PYD and CARD domain-containing protein
  • Target of methylation-induced silencing 1
  • TMS
  • TMS1
  • TMS-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-71853AF350
Alexa Fluor 405 NBP2-71853AF405
Alexa Fluor 488 NBP2-71853AF488
Alexa Fluor 532 NBP2-71853AF532
Alexa Fluor 594 NBP2-71853AF594
Alexa Fluor 647 NBP2-71853AF647
Alexa Fluor 700 NBP2-71853AF700
Alexa Fluor 750 NBP2-71853AF750
Allophycocyanin NBP2-71853APC
Biotin NBP2-71853B
DyLight 350 NBP2-71853UV
DyLight 405 NBP2-71853V
DyLight 488 NBP2-71853G
DyLight 550 NBP2-71853R
DyLight 594 NBP2-71853DL594
DyLight 650 NBP2-71853C
DyLight 680 NBP2-71853FR
DyLight 755 NBP2-71853IR
FITC NBP2-71853F
HRP NBP2-71853H
Janelia Fluor 549 NBP2-71853JF549
Janelia Fluor 646 NBP2-71853JF646
PE NBP2-71853PE
PerCP NBP2-71853PCP

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Bioinformatics Tool for ASC/TMS1 Antibody (NBP2-71853)

Discover related pathways, diseases and genes to ASC/TMS1 Antibody (NBP2-71853). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on ASC/TMS1.

The inflammasome: an inflammation-initiating machine, Novus Biologicals
By Stephanie Melchor The inflammasome is a large, multimeric protein complex found primarily in innate immune cells, which are white blood cells that can attack a wide range of pathogenic threats. Three main elements...  Read full blog post.

Immunity’s flipside: Microglia promote Alzheimer’s pathology during inflammation
By Jamshed Arslan Pharm.D. Microglia are brain's macrophages. In Alzheimer's disease (AD), microglia clear up protein aggregates called amyloid beta plaques. The connection between immune activation and AD is unclea...  Read full blog post.

CARD & NFKB Antibodies for Apoptosis Research
Apoptosis is one of the main types of programmed cell death which involves a cascade of biochemical events leading to specific cell morphology characteristics and ultimately death of cells.Caspases play crucial roles in modulating cellular signaling...  Read full blog post.

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Bioinformatics

Gene Symbol PYCARD