ASC/TMS1 Antibody (1C3D3)

Western Blot: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis using PYCARD mAb against human PYCARD (AA: 1-120) recombinant protein. (Expected MW is 39.2 kDa)

Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis of Hela cells using PYCARD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis of stomach cancer tissues using PYCARD mouse mAb with DAB staining.

Flow Cytometry: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis of HEK293 cells using PYCARD mouse mAb (green) and negative control (red).

Western Blot: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis using PYCARD mAb against HEK293 (1) and PYCARD (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis of GC-7901 cells using PYCARD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

ELISA: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
• Clone: 1C3D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ASC/TMS1 Antibody (1C3D3) Summary

• Immunogen: Purified recombinant fragment of human PYCARD (AA: 1-120) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PYCARD
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
• Theoretical MW: 21.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for ASC/TMS1 Antibody (1C3D3)

• ASC
• ASCMGC10332
• CARD5
• CARD5TMS
• caspase recruitment domain protein 5
• Caspase recruitment domain-containing protein 5
• hASC
• PYCARD
• PYD and CARD domain containing
• PYD and CARD domain-containing protein
• Target of methylation-induced silencing 1
• TMS1
• TMS-1
• TMS1apoptosis-associated speck-like protein containing a CARD

Background

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PYCARD