ASAP1 [p Tyr782] Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: ASAP1 [p Tyr782] Antibody [NBP1-77933] - Analysis of phosphorylated ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when ...read more
Immunohistochemistry: ASAP1 [p Tyr782] Antibody [NBP1-77933] - Used at 20 g/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate intracellular ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.09 mg/ml

Order Details

ASAP1 [p Tyr782] Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 777-787 of mouse ASAP1 protein.
Modification
p Tyr782
Specificity
This antibody is directed against the phosphorylated form of mouse ASAP1 protein at the pY782 residue.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ASAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:4000-1:16000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 20-40 ug/ml
  • Immunohistochemistry-Paraffin 20 ug/ml
Application Notes
Expect a band approximately 130 kDa in size corresponding - phosphorylated ASAP1 protein by western blotting in the appropriate cell lysate or extract. Less than 2.5% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pY782 of ASAP1 protein.

Reactivity Notes

Chimpanzee 100%

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.09 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASAP1 [p Tyr782] Antibody

  • 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activatingprotein
  • AMAP1
  • ARF GTPase-activating protein 1
  • arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
  • ArfGAP with SH3 domain, ankyrin repeat and PH domain 1
  • centaurin, beta 4
  • CENTB4
  • DDEF1ADP-ribosylation factor-directed GTPase-activating protein 1
  • development and differentiation enhancing factor 1
  • Development and differentiation-enhancing factor 1
  • Differentiation-enhancing factor 1
  • KIAA1249DEF-1
  • PAG2
  • PAP
  • PIP2-dependent ARF1 GAP
  • ZG14P

Background

ASAP1 ( Arf-Gap with SH3 domain, ANK repeat and PH domain-containing protein 1) is an Arf (ADP-ribosylation factor) GAP (GTPase-activating protein) that catalyzes the hydrolysis of GTP bound to Arf, a small GTP-binding protein. As a regulator of Arf activity ASAP1 has been shown to regulate cell adhesion and migration, and to be involved in carcinogenesis. Structurally, ASAP1 bears two ankyrin repeats, one Arf-GAP domain, one PH domain, one SH3 domain, and an N-terminal Bin, Amphiphysin and Rvs167 (BAR) domain. Alternative names for ASAP1 include PIP2-dependent ARF1 GAP, ARF GTPase-activating protein 1, development and differentiation-enhancing factor 1, differentiation-enhancing factor 1, PAP, PAG2, AMAP1, ZG14P, CENTB4, DEF-1, DDEF1, and KIAA1249.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ASAP1
Uniprot