Arylsulfatase B/ARSB Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Huh-7 human hepatoma cell line, and human skeletal muscle tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Arylsulfatase B/ARSB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4415) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Arylsulfatase B/ARSB at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Arylsulfatase B/ARSB was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Arylsulfatase B/ARSB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4415) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Arylsulfatase B/ARSB at approximately 59 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Arylsulfatase B/ARSB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4415) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Arylsulfatase B/ARSB Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Arylsulfatase B/ARSB
  Ser37-Met533 (Val376Met)
  Accession # P15848
• Specificity: Detects human Arylsulfatase B/ARSB in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ARSB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Arylsulfatase B/ARSB Antibody [Unconjugated]

• ARSB
• Arylsulfatase B
• ASB
• EC 3.1.6.12
• G4S
• MPS6
• N-acetylgalactosamine-4-sulfatase

Background

ARSB, also known as N-acetylgalactosamine-4-sulfatase, is a lysosomal glycoprotein and one of at least nine human arylsulfatases that catalyze the hydrolysis of sulfate ester bonds. ARSB deficiency is causative of the lysosomal storage disease Mucopolysaccharidosis VI. Human ARSB is cleaved intracellularly into fragments which remain associated with a disulfide linkage. Mature human ARSB (aa 37-533) shares 80% amino acid sequence identity with mouse and rat ARSB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARSB
• Uniprot:
  • P15848()