Arylsulfatase A/ARSA Antibody - Azide and BSA Free

Western Blot: Arylsulfatase A/ARSA Antibody [NBP2-98210] - Anti-Arylsulfatase A/ARSA rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).

Immunoprecipitation: Arylsulfatase A/ARSA Antibody [NBP2-98210] - Mouse Arylsulfatase A/ARSA was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-Mouse Arylsulfatase A/ARSA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Arylsulfatase A/ARSA rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa

• Reactivity: Mu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Arylsulfatase A/ARSA Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Mouse Arylsulfatase A/ARSA (Accession#: NP_033843.2; Met 1-Ser 506)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARSA
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 4-6 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Arylsulfatase A/ARSA Antibody - Azide and BSA Free

• ARSA
• Arylsulfatase A
• ASA
• Cerebroside 3-sulfatase
• Cerebroside-sulfatase
• EC 3.1.6
• EC 3.1.6.8
• MLD

Background

ARSA is encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Multiple alternatively spliced transcript variants, one of which encodes a distinct protein, have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARSA