Artemin Antibody

Western Blot: Artemin Antibody [NBP1-68857] - (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Artemin Antibody Summary

• Description: Novus Biologicals Goat Artemin Antibody (NBP1-68857) is a polyclonal antibody validated for use in WB and ELISA. Anti-Artemin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NSTWRTVDRLS corresponding to internal region according to NP_476501.1, NP_476431.2.
• Epitope: C-NSTWRTVDRLS
• Specificity: This antibody is expected to recognize all reported isoforms (NP_003967.2; NP_476501.1; NP_476431.2). Reported variants represent identical protein: NP_476431.2, NP_001129687.1 Reported variants represent identical protein: NP_003967.2, NP_476432.2
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARTN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 28 kDa band observed in lysates of HEK293, HepG2 and Kelly (PMID: 20118197).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Artemin Antibody

• Artemin
• ARTN
• Enovin
• EVN
• EVNneurotrophic factor
• NBN
• Neublastin

Background

The protein encoded by this gene is a member of the glial cell line-derived neurotophic factor (GDNF) family of ligands which are a group of ligands within the TGF-beta superfamily of signaling molecules. GDNFs are unique in having neurotrophic properties and have potential use for gene therapy in neurodegenrative disease. Artemin has been shown in culture to support the survival of a number of periferal neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons. This protein is a ligand for the RET receptor and uses GFR-alpha 3 as a coreceptor. Four alternatively spliced transcripts have been described, two of which encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Artemin Antibody (NBP1-68857)(1)

Publication using NBP1-68857

• Pandey V, Qian PX, Kang J et al. Artemin stimulates oncogenicity and invasiveness of human endometrial carcinoma cells. Endocrinology 2010-03-01 [PMID: 20118197]

Bioinformatics

• Gene Symbol: ARTN
• Entrez:
  • 9048(Human)
  • 11876(Mouse)
  • 362572(Rat)