ARPC2 Antibody

Western Blot: ARPC2 Antibody [NB100-1037] - Staining of CACO-2 with antibody at 0.5 ug/mL (A), HeLa (B) and Jurkat (C) lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: ARPC2 Antibody [NB100-1037] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry-Paraffin: ARPC2 Antibody [NB100-1037] - Staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.8 ug/mL.

Immunocytochemistry/Immunofluorescence: ARPC2 Antibody [NB100-1037] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu, Rt, Pm, Bv, Ca
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ARPC2 Antibody Summary

• Immunogen: Peptide with sequence C-EMKTITGKTFSSR corresponding to C-Terminus according to NP_005722.1, NP_690601.1.
• Specificity: The reported variants NP_005722.1 and NP_690601.1 represent identical protein.
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARPC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 3 - 5 ug/mL
  • Immunohistochemistry-Paraffin 3 - 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.5 ug/mL
• Application Notes: Western blot: Approx 34-35 kDa band observed in lysates of cell lines CACO-2, HeLa and Jurkat (calculated MW of 34.3 kDa according to NP_005722.1). IHC: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area.
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
  HeLa Whole Cell Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28703125). Primate reactivity reported in scientific literature (PMID: 26349808). Mouse reactivity reported in scientific literature (PMID: 17682067). Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARPC2 Antibody

• actin related protein 2/3 complex, subunit 2 (34 kD)
• actin related protein 2/3 complex, subunit 2, 34kDa
• ARC34actin-related protein 2/3 complex subunit 2
• Arp2/3 complex 34 kDa subunit
• ARP2/3 protein complex subunit 34
• p34-Arc
• PNAS-139

Background

The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARPC2 Antibody (NB100-1037)(6)

Publications using NB100-1037

• Vickery J, Toperzer J, Raab J et al. Synaptopodin is necessary forShigella flexneriintercellular spread bioRxiv 2023-04-26 [PMID: 37163027] (ICC/IF, Human)
• Yuge S, Nishiyama K, Arima Y et al. Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins Nature communications 2022-05-12 [PMID: 35551172] (ICC/IF, Human)
• Leyton-Puig D, Isogai T, Argenzio E et al. Flat clathrin lattices are dynamic actin-controlled hubs for clathrin-mediated endocytosis and signalling of specific receptors Nat Commun 2017-07-13 [PMID: 28703125] (WB, Rat)
• Isogai T, van der Kammen R, Leyton-Puig D et al. Initiation of lamellipodia and ruffles involves cooperation between mDia1 and the Arp2/3 complex. J. Cell. Sci. 2015-09-08 [PMID: 26349808] (WB, Human, Primate)
• Sakata D, Taniguchi H, Yasuda S et al. Impaired T lymphocyte trafficking in mice deficient in an actin-nucleating protein, mDia1. J Exp Med. 2007-09-03 [PMID: 17682067] (WB, Mouse)
  
  Details:
  WB (Fig 5B): primary T cells isolated from adult mouse spleen.
• Welch MD, DePace AH, Verma S et al. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J Cell Biol 1997-07-28 [PMID: 9230079]

Bioinformatics

• Gene Symbol: ARPC2
• Entrez:
  • 10109(Human)
  • 76709(Mouse)
• Uniprot:
  • NP_005722(Human)