ARPC2 Antibody

Images

 
Western Blot: ARPC2 Antibody [NB100-1037] - Staining of CACO-2 with antibody at 0.5 ug/mL (A), HeLa (B) and Jurkat (C) lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: ARPC2 Antibody [NB100-1037] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by ...read more
Immunohistochemistry-Paraffin: ARPC2 Antibody [NB100-1037] - Staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.8 ug/mL.
Immunocytochemistry/ Immunofluorescence: ARPC2 Antibody [NB100-1037] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Bv, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ARPC2 Antibody Summary

Immunogen
Peptide with sequence C-EMKTITGKTFSSR corresponding to C-Terminus according to NP_005722.1, NP_690601.1.
Specificity
The reported variants NP_005722.1 and NP_690601.1 represent identical protein.
Predicted Species
Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ARPC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 3 - 5 ug/mL
  • Immunohistochemistry-Paraffin 3 - 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.5 ug/mL
Application Notes
Western blot: Approx 34-35 kDa band observed in lysates of cell lines CACO-2, HeLa and Jurkat (calculated MW of 34.3 kDa according to NP_005722.1). IHC: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area.
Control
Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
HeLa Whole Cell Lysate
Publications
Read Publications using
NB100-1037 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28703125). Primate reactivity reported in scientific literature (PMID: 26349808). Mouse reactivity reported in scientific literature (PMID: 17682067). Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARPC2 Antibody

  • actin related protein 2/3 complex, subunit 2 (34 kD)
  • actin related protein 2/3 complex, subunit 2, 34kDa
  • ARC34actin-related protein 2/3 complex subunit 2
  • Arp2/3 complex 34 kDa subunit
  • ARP2/3 protein complex subunit 34
  • p34-Arc
  • PNAS-139

Background

The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ARPC2 Antibody (NB100-1037)(5)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Hu, Pm.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(3)
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Mouse
(1)
Rat
(1)
Hu
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ARPC2 Antibody (NB100-1037) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARPC2
Entrez
Uniprot