ARID2 Antibody

Images

 
Western Blot: ARID2 Antibody [NBP1-26615] - Whole cell lysate from HeLa cells. ARID2 was also immunoprecipitated by rabbit anti-ARID2 antibody NBP1-26614.
Immunohistochemistry-Paraffin: ARID2 Antibody [NBP1-26615] - Human breast carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
Immunoprecipitation: ARID2 Antibody [NBP1-26615] - ARID2 IP in MEFs. Image from verified customer review.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

ARID2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1785 and 1835 of human AT rich interactive domain 2 using the numbering given in entry NP_689854.2 (GeneID 196528).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARID2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 5-10 ug/mg lysate
Application Notes
Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-26615 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARID2 Antibody

  • ARID domain-containing protein 2
  • AT rich interactive domain 2 (ARID, RFX-like)
  • BAF200KIAA1557AT-rich interactive domain-containing protein 2
  • BRG1-associated factor 200
  • DKFZp686G052
  • DKFZp779P0222
  • FLJ30619
  • p200
  • Zinc finger protein with activation potential
  • zipzap
  • zipzap/p200

Background

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SWI/SNF complexes possess ATPase and DNA helicase activity that promotes transcriptional activation of genes normally repressed by chromatin structure. ARID2 (AT-rich interactive domain-containing protein 2) is also known as BAF200 and was purified as a factor that associated with PBAF (hSWI/SNF-B). ARID2 is a noncatalytic subunit that is a member of the ARID (AT-rich interactive domain-containing protein) family of DNA binding proteins. In addition to the ARID domain, ARID2 also contains a C2H2-type zinc finger and has been implicated as a transcriptional co-activator for serum response factor for the regulation of cardiac genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-616
Species: Hu, Mu, Rt, Md, Pm
Applications: WB, ChIP, EM, ICC/IF, IP, In vitro, Flow-IC
NBP2-13943
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-01264
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-13883
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
NBP1-88650
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-01345
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
DDX0131P-100
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-47974
Species: Hu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NB100-74648
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB500-179
Species: Hu, Mu, Rt, Po, Ma
Applications: WB, Simple Western, Flow, ICC/IF, IP
NBP1-85841
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88815
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00027044-P01
Species: Hu
AF5414
Species: Hu
Applications: WB, Simple Western
NB500-207
Species: Hu
Applications: WB, ICC/IF, IP
NBP2-33855
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-76788
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-68205
Species: Hu, Mu
Applications: WB, IHC-P, IP
NBP1-30463
Species: Hu, Mu, Ze
Applications: WB, ICC/IF, IP

Publications for ARID2 Antibody (NBP1-26615) (0)

There are no publications for ARID2 Antibody (NBP1-26615).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for ARID2 Antibody (NBP1-26615) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IP.

Reviews using NBP1-26615:
Filter by Applications
IP
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunoprecipitation ARID2 NBP1-26615
Enlarge
reviewed by:
Xiaofeng Wang
IP Mouse 06/13/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedMouse embryonic fibroblasts
SpeciesMouse
LotA-1
CommentsThis antibody works great for both IP and WB in mouse embryonic fibroblasts!

Blocking

Blocking Details10% Non-fat milk in PBST for30 min

Primary Anitbody

Dilution Ratio1:1000; Overnight at 4 degree; in 1% Non-fat milk in PBST

Secondary Antibody

Secondary DescriptionGoat anti-Rabbit IgG-HRP
Secondary Manufacturer Cat#Jackson ImmunoResearch Laboratories
Secondary Concentration1:5000

Details

Detection NotesECL; exposure time: 30 seconds
Immunoprecipitation DetailsDyna-Protein G beads; 30 ul of beads added; antibody incubation O/N at coldroom, then beads were added for another 3h incubation
Elution DescriptionSDS-Loading buffer @100degree for 5 min

Comments

CommentsThis antibody works great for both IP and WB in mouse embryonic fibroblasts!

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ARID2 Antibody (NBP1-26615) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ARID2 Products

Bioinformatics Tool for ARID2 Antibody (NBP1-26615)

Discover related pathways, diseases and genes to ARID2 Antibody (NBP1-26615). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ARID2 Antibody (NBP1-26615)

Discover more about diseases related to ARID2 Antibody (NBP1-26615).
 

Pathways for ARID2 Antibody (NBP1-26615)

View related products by pathway.

Blogs on ARID2

There are no specific blogs for ARID2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Xiaofeng Wang
06/13/2014
Application: IP
Species: Mouse

Bioinformatics

Gene Symbol ARID2
Entrez
Uniprot