ARHGEF16 Antibody

Western Blot: ARHGEF16 Antibody [NBP1-06499] - Whole cell lysate from HeLa and 293T cells.

Immunohistochemistry-Paraffin: ARHGEF16 Antibody [NBP1-06499] - Human ovarian carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).

Immunohistochemistry-Paraffin: ARHGEF16 Antibody [NBP1-06499] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ARHGEF16 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit

Immunohistochemistry-Paraffin: ARHGEF16 Antibody [NBP1-06499] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ARHGEF16 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ARHGEF16 Antibody Summary

• Description: Novus Biologicals Rabbit ARHGEF16 Antibody (NBP1-06499) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human Rho guanine exchange factor (GEF) 16 using the numbering given in entry NP_055263.2 (GeneID 27237).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARHGEF16
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 mcg/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope exposure is recommended. Epitope exposure with citrate buffer or tris-EDTA pH9.0 buffer will enhance staining. Likely to work with frozen sections

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARHGEF16 Antibody

• EPHEXIN4
• Ephexin-4
• GEF16
• NBRRho guanine exchange factor (GEF) 16
• Rho guanine nucleotide exchange factor (GEF) 16
• rho guanine nucleotide exchange factor 16

Background

Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARHGEF16
• Entrez:
  • 27237(Human)
• Uniprot:
  • NP_055263.2(Human)