DAZ1 Antibody (3G10)

Western Blot: DAZ1 Antibody (3G10) [H00001617-M01] - DAZ1 monoclonal antibody (M01), clone 3G10. Analysis of DAZ1 expression in human pancreas.

Immunocytochemistry/Immunofluorescence: DAZ1 Antibody (3G10) [H00001617-M01] - Analysis of monoclonal antibody to DAZ1 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: DAZ1 Antibody (3G10) [H00001617-M01] - Analysis of monoclonal antibody to DAZ1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml.

Sandwich ELISA: DAZ1 Antibody (3G10) [H00001617-M01] - Detection limit for recombinant GST tagged DAZ1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

DAZ1 Antibody (3G10) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DAZ1 (AAH18119, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SSSAAASQGWVLPEGKIVPNTVFVGGIDARMDETEIGSCFGRYGSVKEVKIITNRTGVSKGYGFVSFVNDVDVQKIVGSQIHFHGKKLKLGPAIRKQKLC
• Specificity: DAZ1 - deleted in azoospermia 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DAZ1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against tissue lysate and recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DAZ1 Antibody (3G10)

• deleted in azoospermia 1
• deleted in azoospermia
• FLJ17175
• SPGYDAZdeleted in azoospermia protein 1

Background

This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains three copies of the 10.8 kb repeat. However, no transcripts containing three copies of the RRM domain have been described; thus the RefSeq for this gene contains only two RRM domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DAZ1
• Entrez:
  • 1617(Human)
• OMIM:
  • 400003(Human)
• Uniprot:
  • NP_001006118.2(Human)