Neuronal Pentraxin R/NPTXR Antibody [Unconjugated]

Simple Western lane view shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line and human hippocampus tissue, loaded at 0.2 mg/mL. A specific band was detected for Neuronal Pentraxin R/NPTXR at approximately 66 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human Neuronal Pentraxin R/NPTXR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4414) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Neuronal Pentraxin R/NPTXR was detected in immersion fixed paraffin-embedded sections of Hippocampus using Sheep Anti-Human Neuronal Pentraxin R/NPTXR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4414) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Neuronal Pentraxin R/NPTXR Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Pentraxin R/NPTXR
  Ala24-Lys499
  Accession # O95502
• Specificity: Detects human Neuronal Pentraxin R/NPTXR in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) NPTX2 and rhNPTX1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NPTXR
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuronal Pentraxin R/NPTXR Antibody [Unconjugated]

• Neuronal Pentraxin R
• neuronal pentraxin receptor
• NPR
• NPTXR

Background

NPTXR (Neuronal Pentraxin Receptor; also NPR) is a 65 kDa, type II transmembrane glycoprotein that binds Neuronal Pentraxins 1 and 2. It is expressed on cerebellar Purkinje and granule cells, and hippocampal neurons of the CA1, CA3 and dentate gyrus regions. Human NPTXR is 499 amino acids (aa) in length. It contains a two aa cytoplasmic tail, a 21 aa transmembrane segment, and a 476 aa extracellular region with one Pentraxin domain (aa 295‑499). One alternate start site exists at Met12, and a 55 kDa soluble form (possibly beginning at Gln56) may be generated by proteolytic cleavage. Over aa 24‑499, human NPTXR is 87% aa identical to mouse NPTXR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neuronal Pentraxin R/NPTXR Antibody (AF4414)(4)

Publications using AF4414

• MF Xiao, SE Roh, J Zhou, CC Chien, BP Lucey, MT Craig, LN Hayes, JM Coughlin, FM Leweke, M Jia, D Xu, W Zhou, C Conover Ta, DB Arnold, M Staley, C Jiang, IM Reti, A Sawa, KA Pelkey, CJ McBain, A Savonenko, PF Worley A biomarker-authenticated model of schizophrenia implicating NPTX2 loss of function Science Advances, 2021-11-24;7(48):eabf6935. 2021-11-24 [PMID: 34818031] (Western Blot, Human)
• MF Xiao, D Xu, MT Craig, KA Pelkey, CC Chien, Y Shi, J Zhang, S Resnick, O Pletnikova, D Salmon, J Brewer, S Edland, J Wegiel, B Tycko, A Savonenko, RH Reeves, JC Troncoso, CJ McBain, D Galasko, PF Worley NPTX2 and cognitive dysfunction in Alzheimer's Disease Elife, 2017-03-23;6(0):. 2017-03-23 [PMID: 28440221] (Western Blot, Human)
• Sung-Jin Lee Presynaptic neuronal pentraxin receptor organizes excitatory and inhibitory synapses J. Neurosci, 2016-12-16;0(0):. 2016-12-16 [PMID: 27986928] (Western Blot, Human)
• von Roemeling C, Radisky D, Marlow L, Cooper S, Grebe S, Anastasiadis P, Tun H, Copland J Neuronal pentraxin 2 supports clear cell renal cell carcinoma by activating the AMPA-selective glutamate receptor-4. Cancer Res, 2014-06-24;74(17):4796-810. 2014-06-24 [PMID: 24962026] (IHC, Human)

Bioinformatics

• Gene Symbol: NPTXR
• Uniprot:
  • O95502()