Argininosuccinate Synthase Antibody

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Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of Human Kidney (A) Mouse Liver (B) with antibody at 0.01 ug/mL and Rat Kidney (C) lysate with antibody at 0.03 ug/mL (35 ug protein in RIPA ...read more
Immunocytochemistry/ Immunofluorescence: Argininosuccinate Synthase Antibody [NBP1-00153] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by ...read more
Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153] - (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Argininosuccinate Synthase Antibody [NBP1-00153] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more
Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of A431 (A) and (1ug/ml) NIH3T3(B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Rt, Bv, Mu, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Argininosuccinate Synthase Antibody Summary

Immunogen
Peptide with sequence C-ENPKNQAPPGLYTKTQD corresponding to internal region according to NP_446464.1.
Specificity
The variants represent identical protein (NP_000041.2 and NP_446464.1).
Predicted Species
Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ASS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.03 - 1 ug/mL
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 2 - 4 ug/mL
  • Immunohistochemistry-Paraffin 2 - 4 ug/mL
  • Peptide ELISA Detection limit 1:64000
Application Notes
IP successful usage from BAEC cells is reported in scientific literature (PMID: 18948083).
WB: Approx. 45 kDa band observed in human kidney lysates (calculated MW of 46.5 kDa band according to NP_000041.2 and NP_446464.1). IHC-P: Human kidney shows textured cytoplasm staining in PCT.
Control
Argininosuccinate Synthase Overexpression Lysate
Publications
Read Publications using
NBP1-00153 in the following applications:

  • WB
    1 publication

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 18948083); Rat reactivity reported in scientific literature (PMID: 24113767).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Argininosuccinate Synthase Antibody

  • argininosuccinate synthase 1
  • argininosuccinate synthase
  • argininosuccinate synthetase 1
  • argininosuccinate synthetase
  • ASSCTLN1
  • citrulline-aspartate ligase
  • Citrulline--aspartate ligase
  • EC 6.3.4.5

Background

The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Argininosuccinate Synthase Antibody (NBP1-00153)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Argininosuccinate Synthase Antibody (NBP1-00153) (0)

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol ASS1
Entrez
Uniprot