Arginase 1/ARG1/liver Arginase Antibody

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Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-36936] - Analysis of Pig (A), Mouse (B) and Rat (C) Liver lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-36936] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-36936] - Staining of Human Liver lysate.
Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-36936] - (4ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Arginase 1/ARG1/liver Arginase Antibody Summary

Immunogen
Peptide with sequence CFGLAREGNHKPID corresponding to C-Terminus according to NP_000036.2.
Epitope
C Terminus
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ARG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/ml
  • Immunohistochemistry 2-4ug/ml
  • Immunohistochemistry-Paraffin 2-4ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 37 kDa band observed in human, mouse, rat and porcine liver lysates (calculated MW of 34.7 kDa band according to NP_000036.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Arginase 1/ARG1/liver Arginase Antibody

  • AI
  • ARG1
  • Arginase 1
  • arginase, liver
  • Arginase-1
  • EC 3.5.3.1
  • Liver Arginase
  • Liver-type arginase
  • PGIF
  • Type I Arginase

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic cross reactivity and physiologic function. The type I isoform, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ARG1
Entrez
Uniprot