ARFGEF1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ARFGEF1 Antibody [NBP1-88841] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.

Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NBP1-88841] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ARFGEF1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ARFGEF1 Antibody - BSA Free (NBP1-88841) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-ARFGEF1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARFGEF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ARFGEF1 Protein (NBP1-88841PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ARFGEF1 Antibody - BSA Free

• ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldinA-inhibited)
• ARFGEP1P200
• BIG1p200
• Brefeldin A-inhibited GEP 1
• brefeldin A-inhibited guanine nucleotide-exchange protein 1
• D730028O18Rik
• DKFZP434L057
• p200 ARF guanine nucleotide exchange factor
• p200 ARF-GEP1

Background

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for the guanine-nucleotide exchange activity and also the brefeldin A inhibition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARFGEF1 Antibody (NBP1-88841)(1)

Publication using NBP1-88841

• Christis C, Munro S. The small G protein Arl1 directs the trans-Golgi-specific targeting of the Arf1 exchange factors BIG1 and BIG2. J Cell Biol 2012-02-06 [PMID: 22291037]

Bioinformatics

• Gene Symbol: ARFGEF1