Human APRIL/TNFSF13 ELISA Kit (Colorimetric)

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ELISA: Human APRIL/TNFSF13 ELISA Kit (Colorimetric) [NBP1-82419] - Representative standard curve for human APRIL ELISA. Human APRIL was diluted in serial 2-fold stepsin Sample Diluent. Do not use this standard curve to ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
0.78 - 50.0 ng/mL
Sensitivity
0.4 ng/mL

Order Details

Human APRIL/TNFSF13 ELISA Kit (Colorimetric) Summary

Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human APRIL positive serum. There was no crossreactivity detected.
Standard Curve Range
0.78 - 50.0 ng/mL
Sensitivity
0.4 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
7.1%
Intra-Assay
8.1%
Spike Recovery
The amount of endogenous human APRIL in unspiked serum was subtracted from the spike values. The overall mean recovery was 76%.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TNFSF13

Applications/Dilutions

Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  2. Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human APRIL
  3. Adhesive Films
  4. Biotin-Conjugate anti-human APRIL polyclonal antibody
  5. Stop Solution (1M Phosphoric acid)
  6. Sample Diluent
  7. Human APRIL Standard lyophilized, 100 ng/mL upon reconstitution
  8. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  9. Substrate Solution (tetramethyl-benzidine)
  10. Streptavidin-HRP

Alternate Names for Human APRIL/TNFSF13 ELISA Kit (Colorimetric)

  • A proliferation-inducing ligand
  • APRIL
  • APRILFLJ57090
  • CD256 antigen
  • CD256
  • TALL2
  • TALL-2
  • TNF- and APOL-related leukocyte expressed ligand 2
  • TNF-related death ligand 1
  • TNFSF13
  • TRDL1
  • TRDL-1
  • tumor necrosis factor (ligand) superfamily, member 13
  • tumor necrosis factor ligand superfamily member 13
  • tumor necrosis factor-like protein ZTNF2
  • tumor necrosis factor-related death ligand-1
  • ZTNF2

Background

APRIL (A Proliferation-Inducing Ligand) is a member of the tumor necrosis factor family. APRIL shows high levels of expression in tumors of different origin and low level of expression in normal cells. APRIL shares two TNF receptor family members, TACI and BCMA with another TNF homolog, BlyS/BAFF both of which have been reported to play a role in autoimmune disease and cancer. APRIL appears to play a role in T-independent type II antigen responses and T cell survival, but can also induce proliferation/survival of non lymphoid cells. Local production of APRIL was found in arthritic joints of patients with inflammatory arthritis. Biologically active BlyS/BAFF and APRIL heterotrimers are expressed in patients with systemic immune based rheumatic diseases. A soluble form of the high affinity BCMA receptor has been shown to inhibit the proliferative activity of APRIL in vitro, thus decreasing tumor cell proliferation, while APRIL-transfected cells show an increased rate of tumor growth very directly, suggesting that APRIL is implicated in the regulation of tumor cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol TNFSF13
Entrez