APPL Antibody - BSA Free

Western Blot: APPL Antibody [NBP2-15434] - Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: APPL Antibody [NBP2-15434] - Mouse muscle. APPL1 antibody [N1N2], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: APPL Antibody [NBP2-15434] - AZ521 xenograft, using APPL1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Po, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

APPL Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit APPL Antibody - BSA Free (NBP2-15434) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human APPL. The exact sequence is proprietary.
• Predicted Species: Porcine (98%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APPL1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (89%), Zebrafish (86%), .

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for APPL Antibody - BSA Free

• Adapter protein containing PH domain, PTB domain and leucine zipper motif 1
• adaptor protein containing pH domain, PTB domain and leucine zipper motif 1
• adaptor protein, phosphotyrosine interaction, PH domain and leucine zippercontaining 1
• AKT2 interactor
• APPL
• APPL1
• APPLdip13-alpha
• DCC-interacting protein 13-alpha
• DIP 13 alpha
• DIP13A
• DIP13alpha
• Dip13-alpha
• KIAA1428
• signaling adaptor protein DIP13alpha

Background

The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: APPL1
• Uniprot:
  • Q9UKG1()