APPL Antibody (S05-8D5)

Western Blot: APPL Antibody (S05-8D5) [NBP3-14998] - Western blot detection of APPL in Rat Brain, C6, 3T3, Hela cell lysates using APPL Rabbit mAb (1:1000 diluted). Predicted band size: 80kDa. Observed band size: 80kDa.

Immunohistochemistry-Paraffin: APPL Antibody (S05-8D5) [NBP3-14998] - Immunohistochemistry of APPL in paraffin-embedded Human lung cancer tissue using APPL Rabbit mAb at dilution 1/20

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: S05-8D5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

APPL Antibody (S05-8D5) Summary

• Description: Novus Biologicals Rabbit APPL Antibody (S05-8D5) (NBP3-14998) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human APPL (Uniprot#: Q9UKG1)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: APPL1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000-1/5000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for APPL Antibody (S05-8D5)

• Adapter protein containing PH domain, PTB domain and leucine zipper motif 1
• adaptor protein containing pH domain, PTB domain and leucine zipper motif 1
• adaptor protein, phosphotyrosine interaction, PH domain and leucine zippercontaining 1
• AKT2 interactor
• APPL
• APPL1
• APPLdip13-alpha
• DCC-interacting protein 13-alpha
• DIP 13 alpha
• DIP13A
• DIP13alpha
• Dip13-alpha
• KIAA1428
• signaling adaptor protein DIP13alpha

Background

APPL is encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APPL1