Apolipoprotein E R2/ApoE R2 Antibody

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-76911] - Western blot analysis of ApoER2 on different lysates using anti-ApoER2 antibody at 1/1,000 dilution.Positive control:Lane1: Mouse placentaLane2: SH-SY-5Y

Immunocytochemistry/Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-76911] - ICC staining ApoER2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-76911] - Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ApoER2 antibody. Counter stained with hematoxylin.

Flow Cytometry: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-76911] - Flow cytometric analysis of SH-SY-5Y cells with ApoER2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-76911] - ICC staining ApoER2 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-76911] - ICC staining ApoER2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Apolipoprotein E R2/ApoE R2 Antibody Summary

• Immunogen: Recombinant protein within Human Apolipoprotein E R2/ApoE R2 aa 494-722 / 963. (SwissProt: Q14114 Human; SwissProt: Q924X6 Mouse)
• Localization: Cell membrane. Secreted.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LRP8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500-1:1000
• Theoretical MW: 77 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Apolipoprotein E R2/ApoE R2 Antibody

• ApoER2
• APOER2HSZ75190
• Apolipoprotein E R2
• Apolipoprotein E receptor 2
• low density lipoprotein receptor-related protein 8, apolipoprotein e receptor
• low-density lipoprotein receptor-related protein 8
• Lr8b
• LRP8
• LRP-8
• MCI1

Background

Apolipoprotein E Receptor 2 (ApoER2), also known as LRP8, is a Low-density lipoprotein receptor family member involved in endocytosis and signal transduction. Specifically, ApoER-2 is responsible for the cellular recognition and internalization of ApoE containing lipoproteins, including VLDL and HDL.

ApoER2 is expressed mainly in the brain, placenta, platelets and megakaryocytic cells, but not in the liver. LRP8 mutations can cause myocardial infarction type 1 (MCI1), a condition characterized by the irreversible necrosis of heart muscle secondary to prolonged ischemia. ApoER2 may also play a role in debilitating neurodegenerative diseases such as schizophrenia and Alzheimer's disease.

ApoER2 antibodies are useful tools for studying certian cardiac and neurodegenerative diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRP8