APMAP Antibody (OTI4F6) - Azide and BSA Free

Western Blot: APMAP Antibody (OTI4F6) - Azide and BSA Free [NBP2-72080] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APMAP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APMAP.

Immunohistochemistry: APMAP Antibody (OTI4F6) - Azide and BSA Free [NBP2-72080] - Staining of paraffin-embedded Human prostate tissue using anti-APMAP mouse monoclonal antibody.

Flow Cytometry: APMAP Antibody (OTI4F6) - Azide and BSA Free [NBP2-72080] - Analysis of Hela cells, using anti-APMAP antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: APMAP Antibody (OTI4F6) - Azide and BSA Free [NBP2-72080] - Analysis of extracts (35ug) from 9 different cell lines by using anti-APMAP monoclonal antibody.

Immunohistochemistry: APMAP Antibody (OTI4F6) - Azide and BSA Free [NBP2-72080] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry: APMAP Antibody (OTI4F6) - Azide and BSA Free [NBP2-72080] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry: APMAP Antibody (OTI4F6) - Azide and BSA Free [NBP2-72080] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry: APMAP Antibody (OTI4F6) - Azide and BSA Free [NBP2-72080] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry: APMAP Antibody (OTI4F6) - Azide and BSA Free [NBP2-72080] - Staining of paraffin-embedded Human breast tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry: APMAP Antibody (OTI4F6) - Azide and BSA Free [NBP2-72080] - Staining of paraffin-embedded Human Kidney tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry: APMAP Antibody (OTI4F6) - Azide and BSA Free [NBP2-72080] - Staining of paraffin-embedded Human liver tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry: APMAP Antibody (OTI4F6) - Azide and BSA Free [NBP2-72080] - Staining of paraffin-embedded Human pancreas tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry: APMAP Antibody (OTI4F6) - Azide and BSA Free [NBP2-72080] - Staining of paraffin-embedded Human tonsil using anti-APMAP mouse monoclonal antibody.

CyTOF-ready: APMAP Antibody (OTI4F6) - Azide and BSA Free [NBP2-72080] - Analysis of Jurkat cells, using anti-APMAP antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI4F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

APMAP Antibody (OTI4F6) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human C20orf3(NP_065392) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: APMAP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 46.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for APMAP Antibody (OTI4F6) - Azide and BSA Free

• adipocyte plasma membrane-associated protein
• APMAP
• BSCv
• chromosome 20 open reading frame 3
• Protein BSCv

Background

Exhibits strong arylesterase activity with beta-naphthyl acetate and phenyl acetate. May play a role inadipocyte differentiation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APMAP