APMAP Antibody (OTI4F6)

Western Blot: APMAP Antibody (OTI4F6) [NBP2-01716] - Analysis of extracts (35ug) from 9 different cell lines by using anti-APMAP monoclonal antibody.

Immunohistochemistry-Paraffin: APMAP Antibody (OTI4F6) [NBP2-01716] - Staining of paraffin-embedded Human tonsil using anti-APMAP mouse monoclonal antibody.

Flow Cytometry: APMAP Antibody (OTI4F6) [NBP2-01716] - Analysis of Jurkat cells, using anti-APMAP antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: APMAP Antibody (OTI4F6) [NBP2-01716] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APMAP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APMAP.

Immunohistochemistry-Paraffin: APMAP Antibody (OTI4F6) [NBP2-01716] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry-Paraffin: APMAP Antibody (OTI4F6) [NBP2-01716] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry-Paraffin: APMAP Antibody (OTI4F6) [NBP2-01716] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry-Paraffin: APMAP Antibody (OTI4F6) [NBP2-01716] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry-Paraffin: APMAP Antibody (OTI4F6) [NBP2-01716] - Staining of paraffin-embedded Human breast tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry-Paraffin: APMAP Antibody (OTI4F6) [NBP2-01716] - Staining of paraffin-embedded Human Kidney tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry-Paraffin: APMAP Antibody (OTI4F6) [NBP2-01716] - Staining of paraffin-embedded Human liver tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry-Paraffin: APMAP Antibody (OTI4F6) [NBP2-01716] - Staining of paraffin-embedded Human pancreas tissue using anti-APMAP mouse monoclonal antibody.

Immunohistochemistry-Paraffin: APMAP Antibody (OTI4F6) [NBP2-01716] - Staining of paraffin-embedded Human prostate tissue using anti-APMAP mouse monoclonal antibody.

Flow Cytometry: APMAP Antibody (OTI4F6) [NBP2-01716] - Analysis of Hela cells, using anti-APMAP antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: APMAP Antibody (OTI4F6) [NBP2-01716] - APMAP interacts with LOXL1 & 3 & influences their expression level.A) Protein expression of LOXL3 in eWAT of HFD-fedApmapE1-KO & WT mice; n = 3–4. B)mRNA expression of Lox, Loxl1, & Loxl3 in eWAT; n ≥4. C–E) Interaction of APMAP_E1 with LOXL1(C), LOXL3 (D), & LOX(E) in COS7 cells. Pulldown was performed with flag beads.COS7 cells were transfected with Loxl1/-3-flag or Apmap-flag vectors andHis-Apmap or His-Lox vector. Empty pCMV 5.1 flag & His-LacZserved as controls. One representative pulldown of n ≥3 is shown. F) Scheme of APMAP full-length & truncatedprotein variants. TM, transmembrane region. G, H) Flag beadspulldown with LOXL1-flag (G) & LOXL3-flag(H) & APMAP_E1 & truncated variants (E2, -3, -6) inCOS7 cells. *P ≤ 0.05,**P ≤ 0.01 (Student’st test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28559441), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: APMAP Antibody (OTI4F6) [NBP2-01716] - APMAP interacts with LOXL1 & 3 & influences their expression level.A) Protein expression of LOXL3 in eWAT of HFD-fedApmapE1-KO & WT mice; n = 3–4. B)mRNA expression of Lox, Loxl1, & Loxl3 in eWAT; n ≥4. C–E) Interaction of APMAP_E1 with LOXL1(C), LOXL3 (D), & LOX(E) in COS7 cells. Pulldown was performed with flag beads.COS7 cells were transfected with Loxl1/-3-flag or Apmap-flag vectors andHis-Apmap or His-Lox vector. Empty pCMV 5.1 flag & His-LacZserved as controls. One representative pulldown of n ≥3 is shown. F) Scheme of APMAP full-length & truncatedprotein variants. TM, transmembrane region. G, H) Flag beadspulldown with LOXL1-flag (G) & LOXL3-flag(H) & APMAP_E1 & truncated variants (E2, -3, -6) inCOS7 cells. *P ≤ 0.05,**P ≤ 0.01 (Student’st test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28559441), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, IHC
• Clone: OTI4F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.85 mg/ml

APMAP Antibody (OTI4F6) Summary

• Immunogen: Full length human recombinant protein of human C20orf3(NP_065392) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: APMAP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 46.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.85 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for APMAP Antibody (OTI4F6)

• adipocyte plasma membrane-associated protein
• APMAP
• BSCv
• chromosome 20 open reading frame 3
• Protein BSCv

Background

CHMP1B belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APMAP Antibody (NBP2-01716)(1)

Publication using NBP2-01716

• Pessentheiner AR, Huber K, Pelzmann HJ et al. APMAP interacts with lysyl oxidase-like proteins, and disruption of Apmap leads to beneficial visceral adipose tissue expansion. FASEB J. 2017-05-30 [PMID: 28559441] (WB, Mouse)

Bioinformatics

• Gene Symbol: APMAP
• Entrez:
  • 57132(Human)