Mouse Apelin ELISA Kit (Colorimetric)

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Mouse Apelin - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
125.00 - 8000 pg/mL
Sensitivity
75.00 pg/mL

Order Details

Mouse Apelin ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse APLN in samples. No significant cross-reactivity or interference between Mouse APLN and analogues was observed.
Standard Curve Range
125.00 - 8000 pg/mL
Sensitivity
75.00 pg/mL
Assay Type
Competitive-ELISA
Inter-Assay
CV% < 4.7%
Intra-Assay
CV% < 4.76%
Spike Recovery
86-108%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
APLN

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using NBP2-68235.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100×)
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Wash Buffer (25×)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Mouse Apelin ELISA Kit (Colorimetric)

  • AGTRL1 ligand
  • AGTRL1 Lligand
  • APEL
  • Apelin
  • apelin, AGTRL1 ligand
  • APJ endogenous ligand
  • APLN
  • XNPEP2

Background

Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei and is an endogenous ligand for APJ, a G protein-coupled orphan receptor which is an alternative coreceptor with CD4 for HIV-1. Apelin and APJ are ubiquitously expressed in peripheral tissues, with highest levels reported for heart and lungs, as well several regions within the central nervous system. The actions of apelin remain largely unknown: Apelin inhibits HIV-1 entry in cells coexpressing CD4 and APJ; the oral intake of Apelin in colostrum and breast milk could have a role in the modulation of the immune responses in neonates; more recent studies have also indicated a role for Apelin in the central control of body fluid homeostasis, by influencing AVP release and drinking behavior. In the cardiovascular system several actions of Apelin have been described, including endothelium-dependent vasodilatation, vasoconstriction through direct action on smooth muscle and positive inotropism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol APLN