APBA3/Mint-3 Antibody - BSA Free

Immunohistochemistry-Paraffin: APBA3/Mint-3 Antibody [NBP1-92117] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

APBA3/Mint-3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit APBA3/Mint-3 Antibody - BSA Free (NBP1-92117) is a polyclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QFEALPGDLVGPSPGGAPCPLHIATGHGLASQEIADAHGLLSAEAGRDDLLGLLHCEECPPSQTGPEEPLEPAPRLLQP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APBA3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  APBA3/Mint-3 Protein (NBP1-92117PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for APBA3/Mint-3 Antibody - BSA Free

• Adapter protein X11gamma
• amyloid beta (A4) precursor protein-binding, family A, member 3 (X11-like 2)
• amyloid beta (A4) precursor protein-binding, family A, member 3
• APBA3
• MGC:15815
• MINT3
• mint-3
• Neuronal Munc18-1-interacting protein 3
• Neuron-specific X11L2 protein
• phosphotyrosine-binding/-interacting domain (PTB)-bearing protein
• X11L2
• X11L2amyloid beta A4 precursor protein-binding family A member 3
• X11-like 2 protein

Background

The munc-18 interacting (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three mint isoforms, Mint 1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint 1 and 2 appear to be expressed exclusively in the brain and are found to bind to munc-18, an essential component of the synaptic vesicle fusion machinery. Mint 3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that Mint 3 does not interact with munc-18. Mint 3 has been found to interact with the Alzheimer®s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that Mint 3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer®s disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APBA3