| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06040-50ul | |||
HAF016). A specific band was detected for APBA3 at approximately 96 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human APBA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5689) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for APBA3 at approximately 96 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5689-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92117-25ul | |||
![Western Blot: APBA3/Mint-3 Antibody [NBP3-06040] - Anti-APBA3 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:61 kDa](http://images.novusbio.com/fullsize/APBA3-Mint-3-Antibody-Western-Blot-NBP3-06040-img0001.jpg "Western Blot: APBA3/Mint-3 Antibody [NBP3-06040] - Anti-APBA3 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:61 kDa")
![Immunohistochemistry-Paraffin: APBA3/Mint-3 Antibody [NBP3-06040] - Staining of human APBA3 in human adrenal gland with rabbit polyclonal antibody at 1:300 dilution.](http://images.novusbio.com/fullsize/APBA3-Mint-3-Antibody-Immunohistochemistry-Paraffin-NBP3-06040-img0004.jpg)
![Immunohistochemistry-Paraffin: APBA3/Mint-3 Antibody [NBP3-06040] - Staining of human APBA3 in human kidney with rabbit polyclonal antibody at 1:300 dilution.](http://images.novusbio.com/fullsize/APBA3-Mint-3-Antibody-Immunohistochemistry-Paraffin-NBP3-06040-img0003.jpg)
![Immunoprecipitation: APBA3/Mint-3 Antibody [NBP3-06040] - APBA3 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-APBA3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody: Anti-APBA3 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa Observed band size :72 kDa](http://images.novusbio.com/fullsize/APBA3-Mint-3-Antibody-Immunoprecipitation-NBP3-06040-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: APBA3/Mint-3 Antibody [NBP3-06040] - Staining of APBA3 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06040 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06040_rabbit-polyclonal-apba3-mint-3-antibody-235202514492346.png)
![Immunohistochemistry-Paraffin: APBA3/Mint-3 Antibody [NBP1-92117] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/APBA3-Mint-3-Antibody-Immunohistochemistry-Paraffin-NBP1-92117-img0002.jpg "Immunohistochemistry-Paraffin: APBA3/Mint-3 Antibody [NBP1-92117] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.")
![Western Blot: APBA3/Mint-3 Overexpression Lysate [NBP2-10542] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mint 3.](http://images.novusbio.com/fullsize/Mint-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10542-img0001.jpg "Western Blot: APBA3/Mint-3 Overexpression Lysate [NBP2-10542] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mint 3.")
![Immunoprecipitation:[NBP3-18365]-Detection of human APBA3 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-APBA3 antibody used for IP at 6 µg per reaction. APBA3 was also immunoprecipitated by rabbit anti-APBA3 antibody. For blotting immunoprecipitated APBA3, was used at 0. 4 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/nbp3-18365_rabbit-apba3-mint-3-pab-231202511595043.jpg "Immunoprecipitation:[NBP3-18365]-Detection of human APBA3 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-APBA3 antibody used for IP at 6 µg per reaction. APBA3 was also immunoprecipitated by rabbit anti-APBA3 antibody. For blotting immunoprecipitated APBA3, was used at 0. 4 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
