SET Antibody

Images

 
Western Blot: SET Antibody [NBP1-30888] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody, isoform 2-specifc diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: SET Antibody [NBP1-30888] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SET protein stained by SET antibody, isoform 2-specific diluted at 1:200. Red: ...read more
Immunohistochemistry-Paraffin: SET Antibody [NBP1-30888] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.
Western Blot: SET Antibody [NBP1-30888] - Whole cell lysate treated with SET Antibody, diluted at 1:1000 with 12% SDS PAGE ge.
Western Blot: SET Antibody [NBP1-30888] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 12% SDS PAGE; antibody diluted at 1:10000.
Western Blot: SET Antibody [NBP1-30888] - Sample (30 ug of whole cell lysate) A: Raji B: THP-1 12% SDS PAGE; antibody diluted at 1:10000.
Western Blot: SET Antibody [NBP1-30888] - Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: SET Antibody [NBP1-30888] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Product Details

Summary
Reactivity Hu, Po, Bv, ChSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.959 mg/ml

Order Details

SET Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SET isoform 2. The exact sequence is proprietary.
Localization
Cytoplasm, cytosol, Endoplasmic reticulum, Nucleus, nucleoplasm
Predicted Species
Porcine (100%), Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SET
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-30888 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.959 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SET Antibody

  • 2PP2A
  • HLA-DR-associated protein II
  • I2PP2A
  • I-2PP2A
  • IGAAD
  • Inhibitor of granzyme A-activated DNase
  • inhibitor-2 of protein phosphatase-2A
  • IPP2A2
  • PHAPIITAF-I
  • Phosphatase 2A inhibitor I2PP2A
  • protein phosphatase type 2A inhibitor
  • protein SET
  • SET nuclear oncogene
  • SET translocation (myeloid leukemia-associated)
  • TAF-IBETA
  • Template-activating factor I
  • Template-Activating Factor-I, chromatin remodelling factor

Background

SET belongs to a family of multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. There are two named isoforms produced by alternative splicing : Isoform 1 and Isoform 2. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SET Antibody (NBP1-30888) (0)

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Review for SET Antibody (NBP1-30888) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-30888:
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WB
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Images Ratings Applications Species Date Details
Western Blot SET NBP1-30888
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reviewed by:
Anonymous
WB Human 07/25/2014
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Summary

ApplicationWestern Blot
Sample TestedMCF7 cells
SpeciesHuman
CommentsThe antibody gives one extra band as shown in some images on Novus Bio, but detects the SET domain protein also.

Blocking

Blocking DetailsNon-fat dry milk (5%) in PBS

Primary Anitbody

Dilution Ratio1:1000 in PBS at RT for 3 hours

Secondary Antibody

Secondary DescriptionAnti rabbit IgG (HRP-conjugated)
Secondary Manufacturer Cat#ab6721 (Abcam)
Secondary Concentration1:20K

Details

Detection NotesBio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 1 min

Comments

CommentsThe antibody gives one extra band as shown in some images on Novus Bio, but detects the SET domain protein also.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SET Antibody (NBP1-30888) (0)

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Secondary Antibodies

 

Isotype Controls

Additional SET Products

Bioinformatics Tool for SET Antibody (NBP1-30888)

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Diseases for SET Antibody (NBP1-30888)

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Pathways for SET Antibody (NBP1-30888)

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PTMs for SET Antibody (NBP1-30888)

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Recent Reviews

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5
0
4
1
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0
2
0
1
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Anonymous
07/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol SET