SET Antibody

Western Blot: SET Antibody [NBP1-30888] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody, isoform 2-specifc diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: SET Antibody [NBP1-30888] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SET protein stained by SET antibody, isoform 2-specific diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 um.

Immunohistochemistry-Paraffin: SET Antibody [NBP1-30888] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.

Western Blot: SET Antibody [NBP1-30888] - Whole cell lysate treated with SET Antibody, diluted at 1:1000 with 12% SDS PAGE ge.

Western Blot: SET Antibody [NBP1-30888] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 12% SDS PAGE; antibody diluted at 1:10000.

Western Blot: SET Antibody [NBP1-30888] - Sample (30 ug of whole cell lysate) A: Raji B: THP-1 12% SDS PAGE; antibody diluted at 1:10000.

Western Blot: SET Antibody [NBP1-30888] - Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: SET Antibody [NBP1-30888] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

• Reactivity: Hu, Po, Bv, Ch
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SET Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SET isoform 2. The exact sequence is proprietary.
• Localization: Cytoplasm, cytosol, Endoplasmic reticulum, Nucleus, nucleoplasm
• Predicted Species: Porcine (100%), Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SET
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  SET Lysate (NBP2-64729)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for SET Antibody

• 2PP2A
• HLA-DR-associated protein II
• I2PP2A
• I-2PP2A
• IGAAD
• Inhibitor of granzyme A-activated DNase
• inhibitor-2 of protein phosphatase-2A
• IPP2A2
• PHAPIITAF-I
• Phosphatase 2A inhibitor I2PP2A
• protein phosphatase type 2A inhibitor
• protein SET
• SET nuclear oncogene
• SET translocation (myeloid leukemia-associated)
• TAF-IBETA
• Template-activating factor I
• Template-Activating Factor-I, chromatin remodelling factor

Background

SET belongs to a family of multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. There are two named isoforms produced by alternative splicing : Isoform 1 and Isoform 2. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SET Antibody (NBP1-30888)(1)

Publication using NBP1-30888

• Inturi R, Mun K, Singethan K et al. Human adenovirus infection causes the cellular MKRN1 E3 ubiquitin ligase degradation involving the viral core protein pVII J. Virol. 2017 Nov 15 [PMID: 29142133] (Human)

Review for SET Antibody (NBP1-30888) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 07/25/2014

Summary

• Application: Western Blot
• Sample Tested: MCF7 cells
• Species: Human
• Comments: The antibody gives one extra band as shown in some images on Novus Bio, but detects the SET domain protein also.

Blocking

• Blocking Details: Non-fat dry milk (5%) in PBS

Primary Anitbody

• Dilution Ratio: 1:1000 in PBS at RT for 3 hours

Secondary Antibody

• Secondary Description: Anti rabbit IgG (HRP-conjugated)
• Secondary Manufacturer Cat#: ab6721 (Abcam)
• Secondary Concentration: 1:20K

Details

• Detection Notes: Bio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 1 min

Comments

• Comments: The antibody gives one extra band as shown in some images on Novus Bio, but detects the SET domain protein also.

Bioinformatics

• Gene Symbol: SET