Annexin A3 Antibody (OTI1A5)

Western Blot: Annexin A3 Antibody (1A5) [NBP2-03374] Analysis of extracts (35ug) from 9 different cell lines by using anti-Annexin A3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry-Paraffin: Annexin A3 Antibody (1A5) [NBP2-03374] - Staining of paraffin-embedded Human prostate tissue using anti-Annexin A3 mouse monoclonal antibody.

Flow Cytometry: Annexin A3 Antibody (1A5) [NBP2-03374] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Annexin A3 antibody, and then analyzed by flow cytometry.

Western Blot: Annexin A3 Antibody (1A5) [NBP2-03374] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Annexin A3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Annexin A3.

Immunohistochemistry-Paraffin: Annexin A3 Antibody (1A5) [NBP2-03374] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Annexin A3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Annexin A3 Antibody (1A5) [NBP2-03374] - Staining of paraffin-embedded Human lung tissue using anti-Annexin A3 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI1A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Annexin A3 Antibody (OTI1A5) Summary

• Immunogen: Full length human recombinant protein of human ANXA3 (NP_005130) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANXA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 36.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Annexin A3 Antibody (OTI1A5)

• 35-alpha calcimedin
• Annexin A3
• annexin A3,1,2-cyclic-inositol-phosphate phosphodiesterase
• Annexin III
• annexin-3
• ANXA3
• Calcimedin 35-alpha
• calcimedin 35-alpha
• Lipocortin III
• PAP-III
• Placental anticoagulant protein III
• placental anticoagulant protein III, calcimedin 35-alpha

Background

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANXA3
• Entrez:
  • 306(Human)