Angiopoietin-like Protein 4/ANGPTL4 Antibody

Western Blot: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-19016] - Analysis of ANGPTL4 in 1) Human pancreas 2) Human lung 3) Human lymph node 4) Human adipose and 5) Human breast cancer lysate.

Immunocytochemistry/Immunofluorescence: Angiopoietin-like Protein 4/ANGPTL4 Antibody [NBP2-19016] - Antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Discontinued

Angiopoietin-like Protein 4/ANGPTL4 Antibody Summary

• Immunogen: Synthetic peptide made to an internal sequence of human ANGPTL4 (within residues 150-250) [UniProt Q9BY76].
• Localization: Secreted, vesicles
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANGPTL4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 2 ug/ml
• Application Notes: This ANGPTL4 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is observed ~48 kDa in multiple human tissues and 3T3-L1 cell lysates. In ICC/IF, vesicular staining was observed in U2OS cells.
• Control:

  Human Pancreas Whole Tissue Lysate (Adult Whole Normal)
  Human Lymph Node Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Human and mouse

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Angiopoietin-like Protein 4/ANGPTL4 Antibody

• Angiopoietin like Protein 4
• angiopoietin-like 4
• Angiopoietin-like Protein 4
• ANGPTL4
• ARP4fasting-induced adipose factor
• FIAF
• FIAFhepatic angiopoietin-related protein
• Hepatic fibrinogen/angiopoietin-related protein
• HFARP
• HFARPANGPTL2
• NL2
• peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein
• PGAR
• PGARangiopoietin-related protein 4
• pp1158
• PPARG angiopoietin related protein

Background

Angiopoietin-related protein 4 (ANGPTL4), also known as angiopoietin-like protein 4, hepatic fibrinogen/angiopoietin-related protein, ARP4, HFARP, PGAR, NL2, FIAF, pp1158, and ANGPTL2, stops proliferation, movement, and tubule formation of endothelial cells and helps to prevent vascular leaking. ANGPTL4 is critical for regulation of glucose homeostasis and insulin sensitivity. The ANGPTL4 protein is a hormone that controls lipid metabolism and helps to regulate apoptosis. ANGPTL4 is characterized by hypoxia-induced expression in endothelial cells. During periods of hypoxia, ANGPTL4 is found in high levels in the sub-endothelial extracellular matrix, and the increased presence of ANGPTL4 hinders the adhesion of the endothelial cells. Many researchers believe ANGPTL4 can inhibit tumor motility and invasiveness as well as regulate angiogenesis. ANGPTL4 is a member of the angiopoietin/angiopoientin-like gene family and is found in high levels in the placenta, heart, liver, muscle, pancreas, and lungs. Lower levels of ANGPTL4 can be found in the brain and kidney. Researchers have found that increasing levels of ANGPTL4 in patients with breast cancer can prevent further cancer movement and growth as ANGPTL4 stops the tumor's ability to adhere (PMID: 21860410). Other researchers, however, believe higher levels of ANGPTL4 actually help colorectal cancer metastasize (PMID: 21937683). A third group of research indicates that screening for ANGPTL4 could help determine the origin of clear-cell carcinomas (PMID: 20454689).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANGPTL4
• Entrez:
  • 51129(Human)
  • 57875(Mouse)
• Uniprot:
  • Q9BY76()
  • Q9Z1P8()