Angiopoietin-like Protein 3/ANGPTL3 Antibody [Unconjugated]

Western blot shows lysates of mouse liver tissue and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Angiopoietin-like Protein 3/ANGPTL3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF136) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Angiopoietin-like Protein 3/ANGPTL3 at approximately 35 kDa (major band) and 52 & 63 kDa (minor bands, as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Angiopoietin-like 3 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse Angiopoietin-like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF136) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Angiopoietin-like Protein 3/ANGPTL3 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Angiopoietin-like Protein 3/ANGPTL3
  Ser17-Thr455
  Accession # Q9R182
• Specificity: Detects mouse Angiopoietin-like Protein 3/ANGPTL3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Angiopoietin-1, rhAngiopoietin-2, recombinant mouse Angiopoietin-3, rhAngiopoietin-4, and rhAngiopoietin-like factor/CDT6 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANGPTL3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-like Protein 3/ANGPTL3 Antibody [Unconjugated]

• AGNPT5
• Ang-5
• angiopoietin 5
• Angiopoietin-5
• angiopoietin-like 3
• Angiopoietin-like Protein 3
• angiopoietin-related protein 3
• ANGPT5angiopoietin-5
• ANGPTL3
• FHBL2

Background

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins (1‑3). Mature mouse ANGPTL3 contains an N‑terminal coiled‑coil domain and a C‑terminal fibrinogen-like domain (4). ANGPTL3 is expressed in the liver from early in development through adulthood (4, 5). Full length ANGPTL3 circulates in the plasma as do the proteolytically separated N- and C‑terminal fragments containing the coiled-coil domain and fibrinogen-like domains, respectively (6, 7). ANGPTL3 is found as 70 kDa, 50 kDa, and 32 kDa species and can form weakly associated noncovalent multimers in vitro (5, 6). ANGPTL3 directly inhibits lipoprotein lipase (LPL), an enzyme responsible for hydrolyzing circulating triglycerides (8). This activity requires a putative heparin‑binding motif that is N‑terminal to the coiled‑coil domain (6). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo (6). ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (6‑8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (9). ANGPTL3 expression in vivo is up‑regulated by LXR agonists and down‑regulated by insulin, leptin, and TR beta agonists (10‑12). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice, (7, 8, 11). ANGPTL3 does not bind Tie-1 or Tie-2 but its fibrinogen-like domain interacts with integrin alpha V beta 3 to induce endothelial cell adhesion, migration, and neovascularization (13). ANGPTL3, secreted by fetal liver cells, also promotes the expansion of hematopoietic stem cells (14). Mature mouse ANGPTL3 shares 22%‑30% amino acid (aa) sequence identity with ANGPTL1, 2, 4, 6, and 7. It shares 77% aa sequence identity with human ANGPTL3.

1. Li, C. (2006) Curr. Opin. Lipidol. 17:152.
2. Oike, Y. et al. (2004) Int. J. Hematol. 80:21.
3. Kersten, S. (2005) Biochem. Soc. Transact. 33:1059.
4. Conklin, D. et al. (1999) Genomics 62:477.
5. Ge, H. et al. (2005) J. Lipid Res. 46:1484.
6. Ono, M. et al. (2003) J. Biol. Chem. 278:41804.
7. Koishi, R. et al. (2002) Nat. Genet. 30:151.
8. Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742.
9. Koster, A. et al. (2005) Endocrinology 146:4943.
10. Inaba, T. et al. (2003) J. Biol. Chem. 278:21344.
11. Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080.
12. Fugier, C. et al. (2006) J. Biol. Chem. 281:11553.
13. Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281.
14. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-like Protein 3/ANGPTL3 Antibody (AF136)(9)

Publications using AF136

• Liu, X;Zhang, Y;Han, B;Li, L;Li, Y;Ma, Y;Kang, S;Li, Q;Kong, L;Huang, K;Song, BL;Liu, Y;Wang, Y; Postprandial exercise regulates tissue-specific triglyceride uptake through angiopoietin-like proteins JCI insight 2024-08-22 [PMID: 39171527] (Western Blot, Mouse, Rat)
• Zuo, Y;Zhang, C;Zhou, Y;Li, H;Xiao, W;Herzog, RW;Xu, J;Zhang, J;Chen, YE;Han, R; Liver-specific in vivo base editing of Angptl3 via AAV delivery efficiently lowers blood lipid levels in mice Cell & bioscience 2023-06-15 [PMID: 37322547] (Western Blot, Mouse)
• S Lv, Y Zhou, J Chen, H Yuan, ZN Zhang, B Luan Hepatic ER stress suppresses adipose browning through ATF4-CIRP-ANGPTL3 cascade Cell Reports, 2022-09-27;40(13):111422. 2022-09-27 [PMID: 36170814] (IHC, Western Blot, Mouse)
• F Zhong, S Liu, Y Li, G Li, M Liu, J Wang, W Cui, Y Suo, X Gao ANGPTL3 impacts proteinuria and hyperlipidemia in primary nephrotic syndrome Lipids in Health and Disease, 2022-04-10;21(1):38. 2022-04-10 [PMID: 35399079] (Western Blot, Mouse)
• X Li, Y Zhang, M Zhang, Y Wang GALNT2 regulates ANGPTL3 cleavage in cells and in vivo of mice Sci Rep, 2020-09-30;10(1):16168. 2020-09-30 [PMID: 32999434] (Western Blot, Mouse)
• Federico Oldoni, Haili Cheng, Serena Banfi, Viktoria Gusarova, Jonathan C. Cohen, Helen H. Hobbs ANGPTL8 has both endocrine and autocrine effects on substrate utilization JCI Insight 9/3/2020 [PMID: 32730227]
• Essalmani R, Susan-Resiga D, Chamberland A, Asselin M, Canuel M, Constam D, Creemers J, Day R, Gauthier D, Prat A, Seidah N Furin is the primary in vivo convertase of angiopoietin-like 3 and endothelial lipase in hepatocytes. J Biol Chem, 2013-08-05;288(37):26410-8. 2013-08-05 [PMID: 23918928] (Western Blot, Mouse)
• Gao X, Xu H, Liu H Angiopoietin-like protein 3 regulates the motility and permeability of podocytes by altering nephrin expression in vitro. Biochem. Biophys. Res. Commun., 2010-07-13;399(1):31-6. 2010-07-13 [PMID: 20633534] (Western Blot, Mouse)
• Chou S, Lodish HF Fetal liver hepatic progenitors are supportive stromal cells for hematopoietic stem cells. Proc. Natl. Acad. Sci. U.S.A., 2010-04-12;107(17):7799-804. 2010-04-12 [PMID: 20385801] (ICC, Mouse)

Bioinformatics

• Gene Symbol: ANGPTL3
• Uniprot:
  • Q9R182(Mouse)