Angiopoietin-4 Antibody - BSA Free

Western Blot: Angiopoietin-4 Antibody [NBP1-32607] - Sample (30ug whole cell lysate)A:H1299 B:Hep G2 C:MOLT4 7. 5% SDS PAGE, antibody diluted at 1:500.

Immunohistochemistry-Paraffin: Angiopoietin-4 Antibody [NBP1-32607] - Paraffin-embedded mouse duodenum. Angiopoietin 4 antibody diluted at 1:500.

Immunohistochemistry-Paraffin: Angiopoietin-4 Antibody [NBP1-32607] - Paraffin-embedded DU145 xenograft, using antibody at 1:100 dilution.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Angiopoietin-4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Angiopoietin-4 Antibody - BSA Free (NBP1-32607) is a polyclonal antibody validated for use in IHC and WB. Anti-Angiopoietin-4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Angiopoietin-4. The exact sequence is proprietary.
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANGPT4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Angiopoietin-4 Antibody - BSA Free

• AGP4
• ANG3
• ANG-3
• ANG-4
• ANG4angiopoietin-3
• angiopoietin 4
• Angiopoietin-3
• Angiopoietin-4
• ANGPT4
• dJ824F16.2 (angiopoietin 4)
• MGC138181
• MGC138183

Background

Angiopoietin 3 (ANG3), also called ANG4, might represent counterparts in mouse and human. Based on chromosomal analysis, mouse ANG3 is thought to be an ortholog of human ANG4. Human ANG4 acts as an agonist that binds and activates Tie2, whereas mouse ANG3 is likely to be a Tie2 antagonist, exerting agonist or antagonist activities depending on the cell context. Mouse ANG3 is expressed in various mouse tissues. Mouse angiopoietin 3 is a secreted glycoprotein and member of the angiopoietin family. It has an N terminal coiled-coil domain and a Cbterminal fibrinogen like domain. The coiled coil domains mediate ligand homo oligomerization and the fibrinogen like domains mediate ligand activity. Recombinant mouse angiopoietin 3 has a calculated molecular mass of 58 kDa. Due to glycosylation, the recombinant protein (containing 3 peptides) has an apparent molecular mass of 85-90 kDa in SDS-PAGE under reducing conditions. Mouse angiopoietin 3 shares 47%, 46%, and 54% sequence identity with mouse ANG1, mouse ANG2, and human ANG4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Angiopoietin-4 Antibody (NBP1-32607)(1)

Publication using NBP1-32607

• Barsky M, Merkison J, Hosseinzadeh P, et al. Fetal programming of polycystic ovary syndrome: Effects of androgen exposure on prenatal ovarian development The Journal of steroid biochemistry and molecular biology 2021-01-27 [PMID: 33515680] (WB, Mouse)

Bioinformatics

• Gene Symbol: ANGPT4
• Entrez:
  • 51378(Human)
• Uniprot:
  • Q9Y264()