Angiopoietin-4 Antibodies

CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Angiopoietin‑4 was detected in immersion fixed paraffin-embedded sections of human lung using 10 µg/mL Goat Anti-Human Angiopoietin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF964) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

964-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑4 Monoclonal Antibody (Catalog # MAB9641) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Angiopoietin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF964) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 964-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑4 Monoclonal Antibody (Catalog # MAB9641) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Angiopoietin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF964) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Angiopoietin-4 was detected in immersion fixed paraffin-embedded sections of human kidney using Human Angiopoietin-4 Monoclonal Antibody (Catalog # MAB964) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

964-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑4 Monoclonal Antibody (Catalog # MAB9641) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Angiopoietin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF964) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 964-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑4 Monoclonal Antibody (Catalog # MAB9641) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Angiopoietin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF964) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human Angiopoietin‑4 (Catalog # 964-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑4 Monoclonal Antibody (Catalog # MAB9641) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Angiopoietin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF964) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />