Angiopoietin-1 Antibody (171733) [Unconjugated]

• Reactivity: Hu
• Applications: WB, ELISA(Cap)
• Clone: 171733
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Angiopoietin-1 Antibody (171733) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-1
  Ser20-Phe498
  Accession # Q15389
• Specificity: Detects human Angiopoietin-1 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) Ang‑2, rhAng‑4, rmANGPTL3, or rhANGPTL7.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANGPT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human Angiopoietin-1 Biotinylated Antibody (Catalog number BAF923)
  Standard: Recombinant Human Angiopoietin-1 (Catalog number 923-AN)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-1 Antibody (171733) [Unconjugated]

• AGP1
• AGPT
• Ang1
• ANG-1
• angiopoietin 1
• Angiopoietin-1
• ANGPT1
• KIAA0003

Background

Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are two closely related secreted ligands which bind with similar affinity to Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature (1). Ang-1 cDNA encodes a 498 amino acid (aa) residue precursor protein that contains a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-1 shares approximately 97% and 60% aa sequence identity with mouse Ang‑1 and human Ang‑2, respectively (1, 2). Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization (1, 3, 4). Ang-2 has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context (5).

1. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
2. Davis, S. et al. (1996) Cell 87:1161.
3. Witzenbichler, B. et al. (1998) J. Biol. Chem. 273:18514.
4. Papapetropoulos, A. et al. (1999) Lab. Inest. 79:213.
5. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-1 Antibody (MAB9231)(3)

Publications using MAB9231

• Zheng C, Toth J, Bigwarfe T et al. Non-neutralizing antibodies increase endogenous circulating Ang1 levels MAbs 9/11/2018 [PMID: 30199300]
• Kalomenidis I, Kollintza A, Sigala I, Papapetropoulos A, Papiris S, Light RW, Roussos C Angiopoietin-2 levels are elevated in exudative pleural effusions. Chest, 2006-05-01;129(5):1259-66. 2006-05-01 [PMID: 16685017] (ELISA Development, Human)
• Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1. Eur. J. Clin. Invest., 2003-10-01;33(10):883-90. 2003-10-01 [PMID: 14511360] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: ANGPT1
• Uniprot:
  • Q15389()