Androgen Receptor V7 Antibody (DHTR.V7/9394R)

Immunohistochemistry-Paraffin: Androgen Receptor V7 Antibody (DHTR.V7/9394R) [NBP3-23821] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Androgen Receptor V7 Antibody (DHTR.V7/9394R). HIER: Tris/EDTA, pH9.0, 95°C/45min. 2°CAb: HRP-Poly:30min. DAB:5min.

• Reactivity: Hu
• Applications: IHC
• Clone: DHTR.V7/9394R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Androgen Receptor V7 Antibody (DHTR.V7/9394R) Summary

• Description: Store at 2 to 8C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa600-920) of human Androgen Receptor-protein (Androgen Receptor-V7 specific) (exact sequence is proprietary)
• Localization: Nucleus.
• Specificity: AR V7 belongs to the nuclear hormone receptor family. It contains the nuclear receptor DNA binding domain. Steroid hormone receptors are ligand-activated transcription factors that regulate the expression of eukaryotic genes affecting cell proliferation and differentiation in target tissues. The activity of transcription factors is regulated by binding co-activators and co-repressors. This target is a splice variant of the androgen receptor that lacks the C-terminal androgen-binding site. AR-V7 plays an important role in the occurrence, development and drug resistance of prostate cancer.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2ug/ml
• Application Notes: Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for Androgen Receptor V7 Antibody (DHTR.V7/9394R)

• AIS
• androgen receptor
• AR8
• DHTR
• dihydrotestosterone receptor
• HUMARA
• HYSP1
• KD
• NR3C4
• Nuclear receptor subfamily 3 group C member 4
• SBMA
• SMAX1
• TFM

Background

Androgens have a broad range of effects on the appearance and maintenance of male secondary sexual characteristics. Like other members of the steroid superfamily, the androgen receptor (AR) consists of an amino terminal modulating domain, a central DNA binding domain, a hinge region and a carboxy terminal ligand binding domain. The androgen receptor (AR) is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AR