Androgen Receptor V7 Antibody (DHTR.V7/9125R) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: IHC
• Clone: DHTR.V7/9125R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 700

Androgen Receptor V7 Antibody (DHTR.V7/9125R) [Alexa Fluor® 700] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa600-920) of human Androgen Receptor protein (Androgen Receptor-V7 specific) (exact sequence is proprietary)
• Localization: Nucleus.
• Specificity: AR V7 belongs to the nuclear hormone receptor family. It contains the nuclear receptor DNA binding domain. Steroid hormone receptors are ligand-activated transcription factors that regulate the expression of eukaryotic genes affecting cell proliferation and differentiation in target tissues. The activity of transcription factors is regulated by binding co-activators and co-repressors. This target is a splice variant of the androgen receptor that lacks the C-terminal androgen-binding site. AR-V7 plays an important role in the occurrence, development and drug resistance of prostate cancer.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AR
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Androgen Receptor V7 Antibody (DHTR.V7/9125R) [Alexa Fluor® 700]

• AIS
• androgen receptor
• AR8
• DHTR
• dihydrotestosterone receptor
• HUMARA
• HYSP1
• KD
• NR3C4
• Nuclear receptor subfamily 3 group C member 4
• SBMA
• SMAX1
• TFM

Background

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AR