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Androgen R/NR3C4 Antibody (DHTR/882) [PerCP]

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Product Details

Summary
Reactivity Hu, Mu(-)Species Glossary
Applications Flow
Clone
DHTR/882
Clonality
Monoclonal
Host
Mouse
Conjugate
PerCP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47856AF350
Alexa Fluor 405
NBP2-47856AF405
Alexa Fluor 488
NBP2-47856AF488
Alexa Fluor 532
NBP2-47856AF532
Alexa Fluor 594
NBP2-47856AF594
Alexa Fluor 647
NBP2-47856AF647
Alexa Fluor 700
NBP2-47856AF700
Alexa Fluor 750
NBP2-47856AF750
Allophycocyanin
NBP2-47856APC
Biotin
NBP2-47856B
DyLight 350
NBP2-47856UV
DyLight 405
NBP2-47856V
DyLight 488
NBP2-47856G
DyLight 550
NBP2-47856R
DyLight 594
NBP2-47856DL594
DyLight 650
NBP2-47856C
DyLight 680
NBP2-47856FR
DyLight 755
NBP2-47856IR
FITC
NBP2-47856F
HRP
NBP2-47856H
Janelia Fluor 549
NBP2-47856JF549
Janelia Fluor 646
NBP2-47856JF646
PE
NBP2-47856PE
Unconjugated
NBP2-44788-0.1mg
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44788-0.1mg
IHC-Prediluted
NBP2-48200
Azide and BSA Free
NBP2-47856-0.1mg
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Androgen R/NR3C4 Antibody (DHTR/882) [PerCP] Summary

Immunogen
Recombinant full-length human DHTR protein
Localization
Nuclear
Marker
Marker of Androgen Dependence
Specificity
Recognizes a protein of 110kDa, which is identified as androgen receptor (AR). It reacts with full length, and the newly described A form of the receptor. It does not cross react with estrogen, progesterone, or glucocorticoid receptors. The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed, as a marker of hormone-responsiveness and thus it may be useful in identifying patients likely to benefit from anti-androgen therapy. Anti-androgen receptor has been useful clinically in differentiating morpheaform basal cell carcinoma (mBCC) from desmoplastic trichoepithelioma (DTE) in the skin.This MAb is superb for staining of formalin/paraffin tissues.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AR
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Androgen R/NR3C4 Antibody (DHTR/882) [PerCP]

  • AIS
  • Androgen R
  • androgen receptor
  • AndrogenR
  • AR
  • DHTR
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • NR3C4
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SBMA
  • SMAX1
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Progesterone R/NR3C3 Antibody (4E9) [H00005241-M08] - Analysis of monoclonal antibody to PGR on MCF-7 cell. Antibody concentration 10 ug/ml
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Antibody (33)
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Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).
Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).
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Western Blot: Protein S/PROS1 Antibody [NBP1-87217] - Analysis in human plasma.
Immunohistochemistry-Paraffin: Protein S/PROS1 Antibody [NBP1-87217] - Staining of human colon.
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(3B7)
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Western Blot: PLAG1 Antibody (3B7) [H00005324-M02] - Western Blot detection against Immunogen (36.52 KDa).
Immunohistochemistry: PLAG1 Antibody (3B7) [H00005324-M02] - Left, IHC showing TGFBR3 staining in whole tumor sections (upper panels, scale bars=5mm) and enlargement of selected areas (lower panels, scale bars=100um). Right, quantification of IHC TGFBR3 levels in TGFBR3-PLAG1 positive vs. negative tumors. P (high TGFBR3)=0.002, Fisher's exact test. n=5+34. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-017-01178-z), licensed under a CC-BY licence.
ELISA: PLAG1 Antibody (3B7) [H00005324-M02] - Detection limit for recombinant GST tagged PLAG1 is approximately 0.03ng/ml as a capture antibody.
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Puromycin-sensitive am ...
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Immunocytochemistry/Immunofluorescence: Puromycin-sensitive aminopeptidase/NPEPPS Antibody [NBP2-13669] - Immunofluorescent staining of human cell line A549 shows localization to cytosol.
Immunohistochemistry-Paraffin: Puromycin-sensitive aminopeptidase/NPEPPS Antibody [NBP2-13669] - Staining of human testis shows weak to moderate cytoplasmic positivity in Leydig cells and in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Puromycin-sensitive aminopeptidase/NPEPPS Antibody [NBP2-13669] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
PSAT1 Antibody
NBP1-32920
Western Blot: PSAT1 Antibody [NBP1-32920] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [N1C3].
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Immunohistochemistry-Paraffin: PSAT1 Antibody [NBP1-32920] - NCI-N87 xenograft, using PSAT1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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TMEM37 Antibody
NBP2-47602
Immunocytochemistry/Immunofluorescence: TMEM37 Antibody [NBP2-47602] - Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus, nucleoli fibrillar center & cytosol.
Immunohistochemistry-Paraffin: TMEM37 Antibody [NBP2-47602] - Staining in human kidney and pancreas tissues using anti-TMEM37 antibody. Corresponding TMEM37 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: TMEM37 Antibody [NBP2-47602] - Staining of human kidney shows high expression.
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GR/NR3C1 Antibody
(BuGR2)
NB300-731
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Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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NBP2-50599
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Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
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Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Immunohistochemistry-Paraffin: alcohol dehydrogenase 1C Antibody [NBP2-92824] - Paraffin-embedded human stomach using alcohol dehydrogenase 1C.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 1C Antibody [NBP2-92824] - Paraffin-embedded human esophagus using alcohol dehydrogenase 1C.
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Immunocytochemistry/Immunofluorescence: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Staining Alcohol Dehydrogenase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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alcohol
dehydrogenase 4 Antib
NBP1-90233
Western Blot: alcohol dehydrogenase 4 Antibody [NBP1-90233] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 4 Antibody [NBP1-90233] - Staining in human liver and pancreas tissues using anti-ADH4 antibody. Corresponding ADH4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 4 Antibody [NBP1-90233] - Staining of human liver shows high expression.
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47856AF350
Alexa Fluor 405 NBP2-47856AF405
Alexa Fluor 488 NBP2-47856AF488
Alexa Fluor 532 NBP2-47856AF532
Alexa Fluor 594 NBP2-47856AF594
Alexa Fluor 647 NBP2-47856AF647
Alexa Fluor 700 NBP2-47856AF700
Alexa Fluor 750 NBP2-47856AF750
Allophycocyanin NBP2-47856APC
Biotin NBP2-47856B
DyLight 350 NBP2-47856UV
DyLight 405 NBP2-47856V
DyLight 488 NBP2-47856G
DyLight 550 NBP2-47856R
DyLight 594 NBP2-47856DL594
DyLight 650 NBP2-47856C
DyLight 680 NBP2-47856FR
DyLight 755 NBP2-47856IR
FITC NBP2-47856F
HRP NBP2-47856H
Janelia Fluor 549 NBP2-47856JF549
Janelia Fluor 646 NBP2-47856JF646
PE NBP2-47856PE
PerCP NBP2-47856PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol AR