AMPK beta 1 Antibody

Western Blot: AMPK beta 1 Antibody [H00005564-D01P] - Analysis of PRKAB1 expression in transfected 293T cell line by PRKAB1 polyclonal antibody.Lane 1: PRKAB1 transfected lysate(30.40 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

AMPK beta 1 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: PRKAB1 (NP_006244.2, 1 a.a. - 270 a.a.) full-length human protein. MGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYVCKPEERFRAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI
• Specificity: Reacts with protein kinase, AMP-activated, beta 1 non-catalytic subunit.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKAB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This antibody is reactive against transfected lysate in WB and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AMPK beta 1 Antibody

• 5'-AMP-activated protein kinase beta-1 subunit
• AMP-activated protein kinase beta subunit
• AMPK beta -1 chain
• AMPK beta 1
• AMPK beta 1,5'-AMP-activated protein kinase subunit beta-1
• AMPK subunit beta-1
• AMPK
• AMPKb
• HAMPKb
• MGC17785
• PRKAB1
• protein kinase, AMP-activated, beta 1 non-catalytic subunit
• protein kinase, AMP-activated, noncatalytic, beta-1

Background

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKAB1
• Entrez:
  • 5564(Human)
• Uniprot:
  • NP_006244.2(Human)