AMPK alpha 2 Antibody

Western Blot: AMPK alpha 2 Antibody [NBP2-15381] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: AMPK alpha 2 Antibody [NBP2-15381] - Paraffin-embedded mouse brain, using antibody at 1:500 dilution.

Western Blot: AMPK alpha 2 Antibody [NBP2-15381] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: AMPK alpha 2 Antibody [NBP2-15381] - Staining of Mouse (A) and Rat (B) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: AMPK alpha 2 Antibody [NBP2-15381] - Paraffin-embedded Colon carcinoma, using antibody at 1:500 dilution.

ELISA: AMPK alpha 2 Antibody [NBP2-15381] - ELISA detection of AMPK alpha 2 concentration of 2.5 ug/mL

Product Discontinued

AMPK alpha 2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human AMPK alpha 2. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKAA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for AMPK alpha 2 Antibody

• 5'-AMP-activated protein kinase catalytic subunit alpha-2
• AMPK alpha 2
• AMPK subunit alpha-2
• AMPK2
• AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain
• AMPK-alpha-2 chain
• EC 2.7.11
• EC 2.7.11.1
• PRKAA
• PRKAA2
• protein kinase, AMP-activated, alpha 2 catalytic subunit

Background

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PRKAA2
• Uniprot:
  • P54646()