AMPK alpha 2 Antibody [Agarose Immobilized]

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Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Agarose Immobilized

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Catalog# & Conjugate Size Price

AMPK alpha 2 Antibody [Agarose Immobilized] Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human 5'-AMP-activated Protein Kinase, catalytic Alpha-2 chain using the numbering given in entry AAH69823.1 (GeneID 5563).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKAA2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 1:10-1:500
Application Notes
Useful for Immunoprecipitation 15 -25 mcl of gel slurry per 1 -1 mg of protein lysate or extract.
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB600-397.

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, White-tufted-ear marmoset, Crab-eating macaque, African elephant, Chinese hamster, Naked mole rat and Northern white-cheeked gibbon. Mouse reactivity reported in scientific literature (PMID: 25691571)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.1% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for AMPK alpha 2 Antibody [Agarose Immobilized]

  • 5'-AMP-activated protein kinase catalytic subunit alpha-2
  • AMPK alpha 2
  • AMPK subunit alpha-2
  • AMPK2
  • AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain
  • AMPK-alpha-2 chain
  • EC 2.7.11
  • EC 2.7.11.1
  • PRKAA
  • PRKAA2
  • protein kinase, AMP-activated, alpha 2 catalytic subunit

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-397
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Applications: IP

Publications for AMPK alpha 2 Antibody (NB600-397)(1)

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Secondary Antibodies

 

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Blogs on AMPK alpha 2.

AMPK Alpha 1 and lipid metabolism of adipocytes
AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

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Bioinformatics

Gene Symbol PRKAA2
Entrez