AMPK alpha 2 [p Ser345] Antibody (JJ08-19)

Western Blot: AMPK alpha 2 [p Ser345] Antibody (JJ08-19) [NBP2-67863] - Western blot analysis of AMPK alpha 2 on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat An

Immunocytochemistry/Immunofluorescence: AMPK alpha 2 [p Ser345] Antibody (JJ08-19) [NBP2-67863] - Staining Phospho-AMPK alpha 2(S345) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: AMPK alpha 2 [p Ser345] Antibody (JJ08-19) [NBP2-67863] - Analysis of paraffin-embedded human fetal skeletal muscle tissue with Rabbit anti-AMPK alpha 2 [p Ser345] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/Immunofluorescence: AMPK alpha 2 [p Ser345] Antibody (JJ08-19) [NBP2-67863] - Staining Phospho-AMPK alpha 2(S345) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: AMPK alpha 2 [p Ser345] Antibody (JJ08-19) [NBP2-67863] - Analysis of paraffin-embedded human pancreas tissue using anti-Phospho-AMPK alpha 2(S345) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: AMPK alpha 2 [p Ser345] Antibody (JJ08-19) [NBP2-67863] - Analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-AMPK alpha 2(S345) antibody. Counter stained with hematoxylin.

Product Discontinued

AMPK alpha 2 [p Ser345] Antibody (JJ08-19) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser345 of Human AMPK alpha 2 aa 338-352 / 552. (SwissProt: P54646 Human; SwissProt: Q8BRK8 Mouse; SwissProt: Q09137 Rat)
• Modification: p Ser345
• Localization: Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKAA2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for AMPK alpha 2 [p Ser345] Antibody (JJ08-19)

• 5'-AMP-activated protein kinase catalytic subunit alpha-2
• AMPK alpha 2
• AMPK subunit alpha-2
• AMPK2
• AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain
• AMPK-alpha-2 chain
• EC 2.7.11
• EC 2.7.11.1
• PRKAA
• PRKAA2
• protein kinase, AMP-activated, alpha 2 catalytic subunit

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKAA2