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AMPD2 Antibody

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Western Blot: AMPD2 Antibody [NBP1-33698] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPD2 antibody [N3C3] diluted at 1:1000.
Western Blot: AMPD2 Antibody [NBP1-33698] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPD2 antibody [N3C3] diluted at 1:1000.
Immunohistochemistry-Paraffin: AMPD2 Antibody [NBP1-33698] - Paraffin-embedded rat brain. AMPD2 antibody [N3C3] diluted at 1:500.
Western Blot: AMPD2 Antibody [NBP1-33698] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Rt, Mu, BvSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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AMPD2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human AMPD2. The exact sequence is proprietary.
Predicted Species
Mouse (98%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AMPD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
101 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
AMPD2 Lysate (NBP2-65949)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for AMPD2 Antibody

  • adenosine monophosphate deaminase 2 (isoform L)
  • adenosine monophosphate deaminase 2
  • AMP deaminase 2
  • AMP deaminase isoform L
  • AMPD
  • EC 3.5.4.6

Background

Adenosine monophosphate deaminase-2 (EC 3.5.4.6) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: AMPD1 Antibody [NBP2-24509] - Analysis of human AMPDA1 in mouse skeletal muscle lysate in the 1) absence, 2) presence of immunizing peptide and 3) rat skeletal muscle using NBP2-24509 at 1 ug/ml.
Immunohistochemistry-Paraffin: AMPD1 Antibody [NBP2-24509] - IHC analysis of human skeletal muscle using NBP2-24509 at 10 ug/ml.
(1 Publication)
Species: Hu, Mu, Rt, Bv, Ca, Pm
Applications: WB, IHC-P
PGLYRP2/PGRP-L
Antibody
NBP2-49690
Western Blot: PGLYRP2/PGRP-L Antibody [NBP2-49690] - Total protein from MCF7 cells, human and mouse kidney were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-PGRP-L in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: PGLYRP2/PGRP-L Antibody [NBP2-49690] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PGRP-L at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Species: Hu, Mu
Applications: WB, ICC/IF
AMPD3 Antibody
NBP2-14290
Immunocytochemistry/Immunofluorescence: AMPD3 Antibody [NBP2-14290] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & nuclear membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: AMPD3 Antibody [NBP2-14290] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
BRAP Antibody
NBP1-91705
Western Blot: BRAP Antibody [NBP1-91705] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: BRAP Antibody [NBP1-91705] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.
Immunohistochemistry-Paraffin: BRAP Antibody [NBP1-91705] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
IMPA1 Antibody
NBP1-83107
Western Blot: IMPA1 Antibody [NBP1-83107] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: IMPA1 Antibody [NBP1-83107] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.
Immunohistochemistry-Paraffin: IMPA1 Antibody [NBP1-83107] - Staining of human testis shows strong cytoplasmic and nuclear positivity in seminiferous tubules.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Adenosine
Deaminase/ADA Antibod
NBP1-87404
Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining in human duodenum and liver tissues using anti-ADA antibody. Corresponding ADA RNA-seq data are presented for the same tissues.
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Glutaminyl-peptide
Cyclotransferase/QPCT ...
NBP1-81838
Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in human cell line SK-MEL-30.
Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu, Mu
Applications: WB, IHC, IHC-P

5'-Nucleotidase/CD73 A
NBP1-85740
Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Analysis in human cell line U-87 MG.
Immunocytochemistry/Immunofluorescence: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human cell line LHCN-M2 shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human endometrium shows strong membranous positivity in glandular cells.
(4 Reviews)
(6 Publications)
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
Prostatic Acid
Phosphatase/ACPP Anti ...
AF6240
Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6240) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prostatic Acid Phosphatase/ACPP at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Prostatic Acid Phosphatase/ACPP was detected in immersion fixed paraffin-embedded sections of human prostate using Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6240) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Species: Hu
Applications: WB, ELISA, IHC
ADK Antibody
NBP2-15291
Western Blot: ADK Antibody [NBP2-15291] - Sample (30 ug of whole cell lysate) A: A431 B: Hep G2 10% SDS PAGE adenosine kinase antibody, antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: ADK Antibody [NBP2-15291] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - Paraffin-embedded mouse kidney. Adenosine kinase antibody dilution: 1:500.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Purine Nucleoside
Phosphorylase/PNP Ant ...
NBP1-82541
Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541] - Analysis in human cell lines HEK293 and SK-MEL-30 using Anti-PNP antibody. Corresponding PNP RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Immunocytochemistry/Immunofluorescence: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541] - Staining of human cell line RPTEC TERT1 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Purine Nucleoside Phosphorylase/PNP Antibody [NBP1-82541] - Staining in human epididymis and testis tissues using anti-PNP antibody. Corresponding PNP RNA-seq data are presented for the same tissues.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
HPRG Antibody
[Unconjugated]
AF1905
HPRG Antibody [Unconjugated]
(1 Publication)
Species: Mu
Applications: WB
AMPK alpha 2
Antibody [Unconjugate
AF2850
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat AMPK alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AMPK alpha 2 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
AMPK alpha 2 was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human/Mouse/Rat AMPK alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for AMPK alpha 2 at approximately 63 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat AMPK alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC
AMPK alpha 1
Antibody
NB100-239
Western Blot: AMPK alpha 1 Antibody [NB100-239] - Extracts from 1. bovine aortic endothelial cells, 2. rat aortic smooth muscle cells, 3. HepG2 cells, or 4. human aortic endothelial cells. Antibody used at 2 ug/ml.
Immunohistochemistry-Paraffin: AMPK alpha 1 Antibody [NB100-239] - Human muscle; Antibody used at a dilution of 1:5,000 (0.2ug/ml).
(1 Review)
(12 Publications)
Species: Hu, Rt, Bv
Applications: WB, IHC, IHC-P, IP
AMPK beta 1
Antibody (2C3)
H00005564-M02
Western Blot: AMPK beta 1 Antibody (2C3) [H00005564-M02] - PRKAB1 monoclonal antibody (M02), clone 2C3 Analysis of PRKAB1 expression in HeLa.
Immunocytochemistry/Immunofluorescence: AMPK beta 1 Antibody (2C3) [H00005564-M02] - Analysis of monoclonal antibody to PRKAB1 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: AMPK beta 1 Antibody (2C3) [H00005564-M02] - Detection limit for recombinant GST tagged PRKAB1 is approximately 0.1ng/ml as a capture antibody.
(1 Review)
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF
GDI1 Antibody
NBP1-31528
Western Blot: GDI1 Antibody [NBP1-31528] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: GDI1 Antibody [NBP1-31528] - Sample: Paraffin-embedded mouse lung. GDI1 antibody dilution: 1:500.
Western Blot: GDI1 Antibody [NBP1-31528] - Sample (30 ug of whole cell lysate) A:NIH-3T3 10% SDS PAGE; antibody diluted at 1:1000.
Species: Hu, Mu
Applications: WB, IHC, IHC-P
DBT Antibody
NBP1-89522
Western Blot: DBT Antibody [NBP1-89522] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-89522] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining in human kidney and pancreas tissues using anti-DBT antibody. Corresponding DBT RNA-seq data are presented for the same tissues.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
TNF-alpha
[Unconjugated]
210-TA
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
(19 Reviews)
(492 Publications)
Species: Hu
HPRT Antibody
NBP1-33527
Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: HPRT Antibody [NBP1-33527] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Paraffin-embedded rat colon. HPRT antibody diluted at 1:500.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Butyrylcholinesterase/ ...
NBP2-03523
Western Blot: Butyrylcholinesterase Antibody (1E1) [NBP2-03523] Analysis of extracts (35ug) from 9 different cell lines by using anti-Butyrylcholinesterase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Flow Cytometry: Butyrylcholinesterase Antibody (1E1) [NBP2-03523] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Butyrylcholinesterase antibody, and then analyzed by flow cytometry.
Western Blot: Butyrylcholinesterase Antibody (1E1) [NBP2-03523] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Butyrylcholinesterase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Butyrylcholinesterase.
Species: Hu, Ca
Applications: WB, Flow

Publications for AMPD2 Antibody (NBP1-33698) (0)

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Positive Control Lysate(s)

AMPD2 Knockout 293T Cell Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol AMPD2
Entrez
Uniprot