Aminopeptidase P1/XPNPEP1 Antibody (SAIC-07B-14) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Aminopeptidase P1/XPNPEP1 Antibody (SAIC-07B-14) - Azide and BSA Free [NBP3-20090] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-07B-14 (NBP3-20090) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20090, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody (SAIC-07B-14) - Azide and BSA Free [NBP3-20090] - HeLa(A), Jurkat(B), MCF7(C), HepG2(D), A549(E) and HEK293(F) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-07B-14 (NBP3-20090) at 0.01ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow Cytometry: Aminopeptidase P1/XPNPEP1 Antibody (SAIC-07B-14) - Azide and BSA Free [NBP3-20090] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-07B-14 (NBP3-20090, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, MS
• Clone: SAIC-07B-14
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Aminopeptidase P1/XPNPEP1 Antibody (SAIC-07B-14) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Aminopeptidase P1/XPNPEP1 Antibody (SAIC-07B-14) - Azide and BSA Free (NBP3-20090) is a recombinant monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Peptide "GSLTFEPLTLVPIQTK" derived from Xaa-Pro aminopeptidase 1 conjugated to KLH.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: XPNPEP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Mass Spectrometry
  • Western Blot
• Application Notes: This Ab can be used in immunoMRM.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Aminopeptidase P1/XPNPEP1 Antibody (SAIC-07B-14) - Azide and BSA Free

• Aminoacylproline aminopeptidase
• Aminopeptidase P1
• APP1
• Cytosolic aminopeptidase P
• cytosolic
• EC 3.4.11.9
• SAMP
• xaa-Pro aminopeptidase 1
• XPNPEP1
• XPNPEPLX-prolyl aminopeptidase (aminopeptidase P)-like
• X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
• X-prolyl aminopeptidase 1, soluble

Background

XPNPEP1 is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of any unsubstituted N-terminal amino acid that is adjacent to a penultimate proline residue. As a result of its specificity toward proline, it has been suggested that XPNPEP1 is important in the maturation and degradation of peptide hormones, neuropeptides, and tachykinins, as well as in the digestion of otherwise resistant dietary protein fragments, thereby complementing the pancreatic peptidases. Deficiency of XPNPEP1 results in excretion of large amounts of imino-oligopeptides in urine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: XPNPEP1