Aminopeptidase N/CD13 Antibody

Images

 
Western Blot: Aminopeptidase N/CD13 Antibody [NBP2-33855] - Analysis in human small intestine tissue.
Immunocytochemistry/ Immunofluorescence: Aminopeptidase N/CD13 Antibody [NBP2-33855] - Staining of human cell line BJ shows localization to plasma membrane. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody [NBP2-33855] - Staining in human small intestine and cerebral cortex tissues using anti-ANPEP antibody. Corresponding ANPEP ...read more
Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody [NBP2-33855] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody [NBP2-33855] - Staining of human small intestine shows high expression.
Simple Western: Aminopeptidase N/CD13 Antibody [NBP2-33855] - Simple Western lane view shows a specific band for Aminopeptidase N/CD13 in 0.2 mg/ml of HEL (left) and H. Kidney (right) lysate(s). This experiment was ...read more
Simple Western: Aminopeptidase N/CD13 Antibody [NBP2-33855] - Electropherogram image of the corresponding Simple Western lane view. Aminopeptidase N/CD13 antibody was used at 1:25 dilution on HEL and H. Kidney lysate(s) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Aminopeptidase N/CD13 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PNTLKPDSYRVTLRPYLTPNDRGLYVFKGSSTVRFTCKEATDVIIIHSKKLNYTLSQGHRVVLRGVGGSQPPDIDKTELVEPTEYLVVHLKGSLVKDSQYEMDSEFEGELADDLAGFYRSEYMEGNVRKVVATTQMQA
Specificity
Specificity of human Aminopeptidase N/CD13 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ANPEP
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Simple Western 1:25
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Aminopeptidase N/CD13 Protein (NBP2-33855PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Aminopeptidase N/CD13 Antibody

  • alanyl (membrane) aminopeptidase
  • Alanyl aminopeptidase
  • Aminopeptidase M
  • Aminopeptidase N
  • ANPEP
  • AP-M
  • APN
  • AP-N
  • CD13 antigen
  • CD13
  • CD13APN
  • EC 3.4.11
  • EC 3.4.11.2
  • gp150
  • LAP1
  • Microsomal aminopeptidase
  • Myeloid plasma membrane glycoprotein CD13
  • p150
  • PEPN
  • PEPNhAPN

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ANPEP
Entrez
Uniprot