Aminopeptidase N/CD13 Antibody (SN71-04)

Western Blot: Aminopeptidase N/CD13 Antibody (SN71-04) [NBP2-67696] - Western blot analysis of Aminopeptidase N/CD13 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hour

Immunocytochemistry/Immunofluorescence: Aminopeptidase N/CD13 Antibody (SN71-04) [NBP2-67696] - Staining CD13 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (SN71-04) [NBP2-67696] - Analysis of paraffin-embedded mouse pancreas tissue using anti-CD13 antibody. Counter stained with hematoxylin.

Western Blot: Aminopeptidase N/CD13 Antibody (SN71-04) [NBP2-67696] - Analysis of CD13 on different lysates using anti-CD13 antibody at 1/1,000 dilution. Positive control: Lane 1: THP-1 Lane 2: Human kidney

Immunocytochemistry/Immunofluorescence: Aminopeptidase N/CD13 Antibody (SN71-04) [NBP2-67696] - Staining CD13 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS

Immunocytochemistry/Immunofluorescence: Aminopeptidase N/CD13 Antibody (SN71-04) [NBP2-67696] - Staining CD13 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (SN71-04) [NBP2-67696] - Analysis of paraffin-embedded human breast tissue using anti-CD13 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (SN71-04) [NBP2-67696] - Analysis of paraffin-embedded human kidney tissue using anti-CD13 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (SN71-04) [NBP2-67696] - Analysis of paraffin-embedded human liver tissue using anti-CD13 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (SN71-04) [NBP2-67696] - Analysis of paraffin-embedded human tonsil tissue using anti-CD13 antibody. Counter stained with hematoxylin.

Product Discontinued

Aminopeptidase N/CD13 Antibody (SN71-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Aminopeptidase N/CD13 aa 401-450 / 967. (SwissProt: P15144 Human; SwissProt: P97449 Mouse; SwissProt: P15684 Rat)
• Localization: Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ANPEP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Aminopeptidase N/CD13 Antibody (SN71-04)

• alanyl (membrane) aminopeptidase
• Alanyl aminopeptidase
• Aminopeptidase M
• Aminopeptidase N
• ANPEP
• AP-M
• APN
• AP-N
• CD13 antigen
• CD13
• CD13APN
• EC 3.4.11
• EC 3.4.11.2
• gp150
• LAP1
• Microsomal aminopeptidase
• Myeloid plasma membrane glycoprotein CD13
• p150
• PEPN
• PEPNhAPN

Background

Alanine aminopeptidase (CD13) is expressed on the majority of peripheral blood monocytes and granulocytes. CD13 is also expressed by the majority of acute myeloid leukemias, chronic myeloid leukemias in myeloid blast crisis, a smaller percentage of lymphoid leukemias and myeloid cell lines. CD13 is also found in several types of non hematopoietic cells such as fibroblasts and endothelial cells and in a soluble form in blood plasma. CD13 is not expressed on B cells, T cells, platelets or erythrocytes. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. CD13 plays a role in biologically active peptide metabolism, in the control of growth and differentiation, in phagocytosis and in bactericidal/tumoricidal activities. CD13 also serves as a receptor for human coronaviruses.

CD13 is commonly used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. Therefore, CD13 antibodies are useful tools for cancer and renal disorder research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANPEP