Aminopeptidase N/CD13 Antibody (R3-63)

Immunocytochemistry/Immunofluorescence: Aminopeptidase N/CD13 Antibody (R3-63) [NBP2-23492] - Staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63,, green in A and Rat anti Mouse CD8, clone YTS105.18, red in B. Cis the merged image with nuclei counterstained blue using DAPI. High power

Immunohistochemistry: Aminopeptidase N/CD13 Antibody (R3-63) [NBP2-23492] - Staining can be observed in normal mouse liver (Green). Image from a verified customer review.

Flow Cytometry: Aminopeptidase N/CD13 Antibody (R3-63) [NBP2-23492] - Staining of mouse peripheral blood mononuclear cells with Rat anti Mouse CD13: Alexa Fluo r(R) 488

Immunohistochemistry-Frozen: Aminopeptidase N/CD13 Antibody (R3-63) [NBP2-23492] - Staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody. High power

Immunohistochemistry-Frozen: Aminopeptidase N/CD13 Antibody (R3-63) [NBP2-23492] - Saining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody. Low power

Product Discontinued

Aminopeptidase N/CD13 Antibody (R3-63) Summary

• Immunogen: Mouse intestinal APN
• Specificity: recognises mouse aminopeptidase N (APN), a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells.
• Preparation Method: Spleen cells from immunised mice were fused with cells of the IR983F rat myeloma cell line.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: ANPEP
• Purity: Protein G purified
• Endotoxin Note: <0.01EU/ug

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:50-1:100
  • Functional
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: IHC reported in a verified customer review.

Reactivity Notes

Human reactivity reported in scientific literature (Saare M et al).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Aminopeptidase N/CD13 Antibody (R3-63)

• alanyl (membrane) aminopeptidase
• Alanyl aminopeptidase
• Aminopeptidase M
• Aminopeptidase N
• ANPEP
• AP-M
• APN
• AP-N
• CD13 antigen
• CD13
• CD13APN
• EC 3.4.11
• EC 3.4.11.2
• gp150
• LAP1
• Microsomal aminopeptidase
• Myeloid plasma membrane glycoprotein CD13
• P150
• PEPN
• PEPNhAPN

Background

Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aminopeptidase N/CD13 Antibody (NBP2-23492)(1)

Publication using NBP2-23492

• Saare M. Molecular Profiling of Endometriotic Lesions and Endometria of Endometriosis Patients Thesis 2016-03-16 (FLOW, Human)
  
  Details:
  This reference used the PE version of NBP2-23492.

Bioinformatics

• Gene Symbol: ANPEP