Home » Aminopeptidase N/CD13 » Aminopeptidase N/CD13 Antibodies » Aminopeptidase N/CD13 Antibody (B-F10) [DyLight 680]

Aminopeptidase N/CD13 Antibody (B-F10) [DyLight 680]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-Fr
Clone
B-F10
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 680

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47891AF350
Alexa Fluor 405
NBP2-47891AF405
Alexa Fluor 488
NBP2-47891AF488
Alexa Fluor 532
NBP2-47891AF532
Alexa Fluor 594
NBP2-47891AF594
Alexa Fluor 647
NBP2-47891AF647
Alexa Fluor 700
NBP2-47891AF700
Alexa Fluor 750
NBP2-47891AF750
Allophycocyanin
NBP2-47891APC
Allophycocyanin/Cy7
NBP2-47891APCCY7
Biotin
NBP2-47891B
DyLight 350
NBP2-47891UV
DyLight 405
NBP2-47891V
DyLight 488
NBP2-47891G
DyLight 550
NBP2-47891R
DyLight 594
NBP2-47891DL594
DyLight 650
NBP2-47891C
DyLight 755
NBP2-47891IR
FITC
NBP2-47891F
HRP
NBP2-47891H
Janelia Fluor 549
NBP2-47891JF549
Janelia Fluor 646
NBP2-47891JF646
PE
NBP2-47891PE
PE/Atto594
NBP2-47891PEATT594
PE/Cy5.5
NBP2-47891PECY55
PE/Cy7
NBP2-47891PECY7
PerCP
NBP2-47891PCP
Unconjugated
NBP2-44680-0.1mg
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44680-0.1mg
Azide and BSA Free
NBP2-47891-0.1mg
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Aminopeptidase N/CD13 Antibody (B-F10) [DyLight 680] Summary

Immunogen
KG-1 myeloid cells
Localization
Cell Surface
Marker
Myeloid Cell Marker
Specificity
Recognizes an integral membrane glycoprotein of 150kDa, identified as CD13 (also known as aminopeptidase-N). The CD13 antigen is present on most cells of myeloid origin including granulocytes, monocytes, mast cells, and GM-progenitor cells. It is also expressed by the majority of AML, CML in myeloid blast crisis, and in a smaller fraction of lymphoid leukemias. CD13 is absent from normal lymphocytes, platelets and erythrocytes. CD13 is also present on fibroblasts; endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining bile duct canaliculi. CD13 is identical to aminopeptidase N (APN), a prominent membrane-bound metalloprotease present on the surface of intestinal brush border and renal tubules. CD13 plays a role in metabolism of biologically active peptides, in phagocytosis, and in bactericidal/tumoricidal activities. It also serves as a receptor for human coronaviruses (HCV). The lineage-restricted pattern of expression of CD13 within the hemopoietic compartment suggests that it may be important in myeloid cell differentiation.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ANPEP
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Aminopeptidase N/CD13 Antibody (B-F10) [DyLight 680]

  • alanyl (membrane) aminopeptidase
  • Alanyl aminopeptidase
  • Aminopeptidase M
  • Aminopeptidase N
  • ANPEP
  • AP-M
  • APN
  • AP-N
  • CD13 antigen
  • CD13
  • CD13APN
  • EC 3.4.11
  • EC 3.4.11.2
  • gp150
  • LAP1
  • Microsomal aminopeptidase
  • Myeloid plasma membrane glycoprotein CD13
  • P150
  • PEPN
  • PEPNhAPN

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-67717
Western Blot: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of CD10 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
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NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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DPPIV/CD26
Antibody [Unconjugate
AF1180
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14.7)
NB600-1071
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AF1730
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Myeloperoxidase/MPO An ...
AF3667
Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line, human neutrophil cells, and mouse spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Myeloperoxidase/MPO at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Myeloperoxidase/MPO at approximately 67 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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Glutaminyl-peptide
Cyclotransferase/QPCT ...
NBP1-81838
Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining in human adrenal gland and liver tissues using anti-QPCT antibody. Corresponding QPCT RNA-seq data are presented for the same tissues.
Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in control (vector only transfected HEK293T lysate) and QPCT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in human cell line SK-MEL-30.
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Aminopeptidase
N/CD13 Antibody (B-F1 ...
NBP2-47891FR
Aminopeptidase N/CD13 Antibody (B-F10) [DyLight 680]
Species: Hu
Applications: Flow, IHC, IHC-Fr

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47891AF350
Alexa Fluor 405 NBP2-47891AF405
Alexa Fluor 488 NBP2-47891AF488
Alexa Fluor 532 NBP2-47891AF532
Alexa Fluor 594 NBP2-47891AF594
Alexa Fluor 647 NBP2-47891AF647
Alexa Fluor 700 NBP2-47891AF700
Alexa Fluor 750 NBP2-47891AF750
Allophycocyanin NBP2-47891APC
Allophycocyanin/Cy7 NBP2-47891APCCY7
Biotin NBP2-47891B
DyLight 350 NBP2-47891UV
DyLight 405 NBP2-47891V
DyLight 488 NBP2-47891G
DyLight 550 NBP2-47891R
DyLight 594 NBP2-47891DL594
DyLight 650 NBP2-47891C
DyLight 680 NBP2-47891FR
DyLight 755 NBP2-47891IR
FITC NBP2-47891F
HRP NBP2-47891H
Janelia Fluor 549 NBP2-47891JF549
Janelia Fluor 646 NBP2-47891JF646
PE NBP2-47891PE
PE/Atto594 NBP2-47891PEATT594
PE/Cy5.5 NBP2-47891PECY55
PE/Cy7 NBP2-47891PECY7
PerCP NBP2-47891PCP
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Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

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Bioinformatics

Gene Symbol ANPEP