Aminopeptidase N/CD13 Antibody (986025) [Unconjugated]

Western blot shows lysates of human prostate tissue, human liver tissue, HepG2 human hepatocellular carcinoma cell line, and PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB38151) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Aminopeptidase N/CD13 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Aminopeptidase N/CD13 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB38151) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to glandular cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 986025
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Aminopeptidase N/CD13 Antibody (986025) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase N/CD13
  Lys69-Lys967
  Accession # AAA51719
• Specificity: Detects human Aminopeptidase N/CD13 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunohistochemistry 0.5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aminopeptidase N/CD13 Antibody (986025) [Unconjugated]

• alanyl (membrane) aminopeptidase
• Alanyl aminopeptidase
• Aminopeptidase M
• Aminopeptidase N
• ANPEP
• AP-M
• APN
• AP-N
• CD13 antigen
• CD13
• CD13APN
• EC 3.4.11
• EC 3.4.11.2
• gp150
• LAP1
• Microsomal aminopeptidase
• Myeloid plasma membrane glycoprotein CD13
• p150
• PEPN
• PEPNhAPN

Background

The human ANPEP gene encodes aminopeptidase N (APN), which is also known as microsomal aminopeptidase, alanyl aminopeptidase, aminopeptidase M, CD13, or membrane protein p161 (1‑3). The deduced amino acid sequence of human APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). The amino acid sequence of human APN is 78% and 77% identical to that of rat and mouse, respectively. Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (3).

1. Olsen, J. et al. (1988) FEBS Lett. 238:307.
2. Look, A.T. et al. (1989) J. Clin. Invest. 83:1299.
3. Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 289, Academic Press, San Diego.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • AAA51719()