Aminopeptidase N/CD13 Antibody (498001) [Unconjugated]

Simple Western lane view shows lysates of human small intestine tissue and human prostate tissue, loaded at 0.5 mg/mL. A specific band was detected for Aminopeptidase N/CD13 at approximately 204 kDa (as indicated) using 40 µg/mL of Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB3815). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Aminopeptidase N/CD13 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 25 µg/mL Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB3815) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of human kidney tissue and human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB3815) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Aminopeptidase N/CD13 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Aminopeptidase N/CD13 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB3815) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to blood vessels. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Aminopeptidase N/CD13 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB3815) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF
• Clone: 498001
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Aminopeptidase N/CD13 Antibody (498001) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase N/CD13
  Lys69-Lys967
  Accession # AAA51719
• Specificity: Detects human Aminopeptidase N/CD13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Aminopeptidase N is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANPEP

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL
  • Simple Western 40 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Immunocytochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aminopeptidase N/CD13 Antibody (498001) [Unconjugated]

• alanyl (membrane) aminopeptidase
• Alanyl aminopeptidase
• Aminopeptidase M
• Aminopeptidase N
• ANPEP
• AP-M
• APN
• AP-N
• CD13 antigen
• CD13
• CD13APN
• EC 3.4.11
• EC 3.4.11.2
• gp150
• LAP1
• Microsomal aminopeptidase
• Myeloid plasma membrane glycoprotein CD13
• p150
• PEPN
• PEPNhAPN

Background

The human ANPEP gene encodes aminopeptidase N (APN), which is also known as microsomal aminopeptidase, alanyl aminopeptidase, aminopeptidase M, CD13, or membrane protein p161 (1‑3). The deduced amino acid sequence of human APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). The amino acid sequence of human APN is 78% and 77% identical to that of rat and mouse, respectively. Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (3).

1. Olsen, J. et al. (1988) FEBS Lett. 238:307.
2. Look, A.T. et al. (1989) J. Clin. Invest. 83:1299.
3. Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 289, Academic Press, San Diego.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aminopeptidase N/CD13 Antibody (MAB3815)(5)

Publications using MAB3815

• Q Ma, Z Zhao, AP Sagare, Y Wu, M Wang, NC Owens, PB Verghese, J Herz, DM Holtzman, BV Zlokovic Blood-brain barrier-associated pericytes internalize and clear aggregated amyloid-?42 by LRP1-dependent apolipoprotein E isoform-specific mechanism Mol Neurodegener, 2018;13(1):57. 2018 [PMID: 30340601] (IHC-P, Human)
• EA Winkler, H Birk, JK Burkhardt, X Chen, JK Yue, D Guo, WC Rutledge, GF Lasker, C Partow, T Tihan, EF Chang, H Su, H Kim, BP Walcott, MT Lawton Reductions in brain pericytes are associated with arteriovenous malformation vascular instability J. Neurosurg., 2018;0(0):1-11. 2018 [PMID: 29303444] (IHC-P, Human)
• X Chen, Y Yuan, Q Wang, F Xie, D Xia, X Wang, Y Wei, T Xie Post-Translational Modification of Adiponectin Affects Lipid Accumulation, Proliferation and Migration of Vascular Smooth Muscle Cells Cell. Physiol. Biochem., 2017;43(1):172-181. 2017 [PMID: 28848139] (Western Blot, Human)
• S Å Urga, MP Nanut, J Kos, J Saboti? Fungal lectin MpL enables entry of protein drugs into cancer cells and their subcellular targeting Oncotarget, 2017;8(16):26896-26910. 2017 [PMID: 28460472] (Co-Immunoprecipitation, Human)
• Emi Y, Harada Y, Sakaguchi M Involvement of a di-leucine motif in targeting of ABCC1 to the basolateral plasma membrane of polarized epithelial cells. Biochem Biophys Res Commun, 2013;441(1):89-95. 2013 [PMID: 24129190] (IHC, Human)

Bioinformatics

• Gene Symbol: ANPEP
• Uniprot:
  • AAA51719()