alpha Tubulin Antibody (961216) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human/Mouse/Rat a Tubulin Monoclonal Antibody (Catalog # MAB9344) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a Tubulin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

a Tubulin was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human/Mouse/Rat a Tubulin Monoclonal Antibody (Catalog # MAB9344) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

alpha Tubulin was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human/Mouse/Rat alpha Tubulin Monoclonal Antibody (Catalog # MAB9344) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, ICC/IF
• Clone: 961216
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

alpha Tubulin Antibody (961216) [Unconjugated] Summary

• Immunogen: Human TUBA1A synthetic peptide
  Accession # Q71U36
• Specificity: Detects human alpha Tubulin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 0.05 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha Tubulin Antibody (961216) [Unconjugated]

• alpha Tubulin
• Alpha-Tubulin 3
• B-ALPHA-1
• FLJ25113
• hum-a-tub1
• hum-a-tub2
• LIS3
• TUBA1A
• TUBA3
• tublin
• tubulin alpha-1A chain
• Tubulin alpha-3 chain
• Tubulin B-Alpha-1
• tubulin, alpha 1a
• tubulin, alpha 3
• tubulin, alpha, brain-specific

Background

Tubulin alpha-1A chain (TUBA1A), also known as Alpha-tubulin 3, is the principal alpha tubulin in morphologically differentiated neurons. Alpha tubulin dimerizes with beta Tubulin to form microtubules. Microtubules mediate transport of proteins and endosomes within cells, and TUBA1A has been shown to interact with metabotropic glutamate receptor 7 (receptor trafficking), synuclein alpha (synaptic plasticity), and N-syndecan (neurite outgrowth).  This gene is mutated in malformations of cortical development, including Lissencephaly resulting in microcephaly, developmental delay and early-onset epileptic seizures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha Tubulin Antibody (MAB9344)(12)

Publications using MAB9344

• Bermúdez-Abreut, E;Bergado Báez, G;Fundora-Barrios, T;Arencibia-Perezleo, J;Medinilla, AL;Chao, L;Sánchez Ramírez, B; HER1 (EGFR) and/or HER2 inclusion potentiates the antitumor effect elicited by a HER3-specific monovalent vaccine Molecular cancer therapeutics 2025-04-16 [PMID: 40237095] (Western Blot, Human)
• Schröder, LC;Hüttermann, L;Kliesow Remes, A;Voran, JC;Hille, S;Sommer, W;Lutter, G;Warnecke, G;Frank, D;Schade, D;Müller, OJ; AAV library screening identifies novel vector for efficient transduction of human aorta Gene therapy 2024-12-18 [PMID: 39695327] (Western Blot, Human)
• Schröder, LC;Hüttermann, L;Kliesow Remes, A;Voran, JC;Hille, S;Sommer, W;Lutter, G;Warnecke, G;Frank, D;Schade, D;Müller, OJ; AAV library screening identifies novel vector for efficient transduction of human aorta Gene therapy 2024-12-18 [PMID: 39695327] (Western Blot)
• Zhou, L;Roth, M;Papakonstantinou, E;Tamm, M;Stolz, D; Expression of glucocorticoid receptor and HDACs in airway smooth muscle cells is associated with response to steroids in COPD Respiratory research 2024-05-29 [PMID: 38812021] (Westen Blot, Human)
• Zhou, L;Fang, L;Tamm, M;Stolz, D;Roth, M; Extracellular Heat Shock Protein 70 Increases the Glucocorticoid Receptor and Dual-Specificity Phosphatase 1 via Toll-like Receptor 4 and Attenuates Inflammation in Airway Epithelial Cells International journal of molecular sciences 2023-07-20 [PMID: 37511459] (Western Blot, Human)
• S Piao, I Lee, S Kim, H Park, H Nagar, SJ Choi, GH Vu, M Kim, EO Lee, BH Jeon, DW Kim, Y Seo, CS Kim CRIF1 siRNA-Encapsulated PLGA Nanoparticles Suppress Tumor Growth in MCF-7 Human Breast Cancer Cells International Journal of Molecular Sciences, 2023-04-18;24(8):. 2023-04-18 [PMID: 37108616] (Western Blot, Human, Mouse)
• M Pappadà, O Bonuccelli, M Buratto, R Fontana, M Sicurella, A Caproni, S Fuselli, A Benazzo, R Bertorelli, V De Sanctis, P Cavallerio, V Simioni, V Tugnoli, F Salvatori, P Marconi Suppressing gain-of-function proteins via CRISPR/Cas9 system in SCA1 cells Scientific Reports, 2022-11-24;12(1):20285. 2022-11-24 [PMID: 36434031] (Western Blot, Human)
• R Rathnasing, S Jangra, C Ye, A Cupic, G Singh, C Martínez-R, LCF Mulder, T Kehrer, S Yildiz, A Choi, ST Yeung, I Mena, V Gillespie, J De Vrieze, S Aslam, D Stadlbauer, DA Meekins, CD McDowell, V Balaraman, MJ Corley, JA Richt, BG De Geest, L Miorin, PVI study, F Krammer, L Martinez-S, V Simon, A García-Sas, M Schotsaert Characterization of SARS-CoV-2 Spike mutations important for infection of mice and escape from human immune sera Nature Communications, 2022-07-07;13(1):3921. 2022-07-07 [PMID: 35798721] (Western Blot, African Green Monkey)
• S Piao, I Lee, SA Jin, S Kim, H Nagar, SJ Choi, BH Jeon, CS Kim SIRT1 Activation Attenuates the Cardiac Dysfunction Induced by Endothelial Cell-Specific Deletion of CRIF1 Biomedicines, 2021-01-08;9(1):. 2021-01-08 [PMID: 33430144] (Western Blot, Mouse)
• DM Tufa, T Shank, AM Yingst, GD Trahan, S Shim, J Lake, R Woods, K Jones, MR Verneris Prolactin Acts on Myeloid Progenitors to Modulate SMAD7 Expression and Enhance Hematopoietic Stem Cell Differentiation into the NK Cell Lineage Sci Rep, 2020-04-14;10(1):6335. 2020-04-14 [PMID: 32286456] (Western Blot, Human)
• MT Kuo, Y Long, WB Tsai, YY Li, HHW Chen, LG Feun, N Savaraj Collaboration Between RSK-EphA2 and Gas6-Axl RTK Signaling in Arginine Starvation Response That Confers Resistance to EGFR Inhibitors Transl Oncol, 2019-12-27;13(2):355-364. 2019-12-27 [PMID: 31887630] (Western Blot, Human)
• Raby A, Gonzalez-Mateo G, Williams A, Topley N, Fraser D, Lopez-Cabrera M, Labeta M Targeting Toll-like receptors with soluble Toll-like receptor 2 prevents peritoneal dialysis solution-induced fibrosis. Kidney Int, 2018-05-31;94(2):346-362. 2018-05-31 [PMID: 29861057] (Western Blot, Human)

Bioinformatics

• Uniprot:
  • Q71U36()