alpha Tubulin 4a Antibody

Western Blot: alpha Tubulin 4a Antibody [NBP1-31392] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: HeLa D: Molt-4 10% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: alpha Tubulin 4a Antibody [NBP1-31392] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using alpha Tubulin 4a antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with red at 1:2000.

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP1-31392] - Paraffin-embedded mouse prostate. alpha Tubulin 4a antibody dilution: 1:500.

Western Blot: alpha Tubulin 4a Antibody [NBP1-31392] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: alpha Tubulin 4a Antibody [NBP1-31392] - Analysis of HeLa, using NBP1-31392 at 1:200 dilution.

Product Discontinued

alpha Tubulin 4a Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 4a. The exact sequence is proprietary.
• Predicted Species: Bovine (100%), Sheep (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TUBA4A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Xenopus laevis (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7)
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for alpha Tubulin 4a Antibody

• alpha 1 (testis specific)
• alpha Tubulin 4a
• Alpha-tubulin 1
• FLJ30169
• H2-ALPHA
• Testis-specific alpha-tubulin
• TUBA1
• TUBA4A
• Tubulin alpha-1 chain
• tubulin alpha-4A chain
• Tubulin H2-alpha
• tubulin, alpha 1
• tubulin, alpha 4a

Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for alpha Tubulin 4a Antibody (NBP1-31392)(2)

Publications using NBP1-31392

• Ehlerding EB, England CG, Jiang D et al. CD38 as a PET imaging target in lung cancer. Molecular Pharmaceutics 2017-06-07 [PMID: 28573863] (WB, ICC/IF, Human)
• Hwang-Verslues WW, Chang PH, Wei PC et al. miR-495 is upregulated by E12/E47 in breast cancer stem cells, and promotes oncogenesis and hypoxia resistance via downregulation of E-cadherin and REDD1. Oncogene 2011-05-01 [PMID: 21258409] (WB)

Bioinformatics

• Gene Symbol: TUBA4A