Western Blot: alpha Tubulin 4a Antibody [NBP1-31392] - A431 (lane A) and Molt-4 (lane B), with 10% SDS PAGE, diluted at 1:1000.
Western Blot: alpha Tubulin 4a Antibody [NBP1-31392] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody [NBP1-31392] - Paraformaldehyde-fixed U2OS, using alpha Tubulin 4a antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an ...read more
Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP1-31392] - Paraffin-embedded H661 xenograft, using antibody at 1:100 dilution.
50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-31392 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Immunogen affinity purified
Alternate Names for alpha Tubulin 4a Antibody
alpha 1 (testis specific)
Tubulin alpha-1 chain
tubulin alpha-4A chain
tubulin, alpha 1
tubulin, alpha 4a
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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