alpha Tubulin 4a Antibody

Images

 
Western Blot: alpha Tubulin 4a Antibody [NBP1-31392] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: HeLa D: Molt-4 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody [NBP1-31392] - Analysis of HeLa, using NBP1-31392 at 1:200 dilution.
Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP1-31392] - Paraffin-embedded mouse prostate. alpha Tubulin 4a antibody dilution: 1:500.
Western Blot: alpha Tubulin 4a Antibody [NBP1-31392] - A431 (lane A) and Molt-4 (lane B), with 10% SDS PAGE, diluted at 1:1000.
Western Blot: alpha Tubulin 4a Antibody [NBP1-31392] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin 4a Antibody [NBP1-31392] - Paraformaldehyde-fixed U2OS, using alpha Tubulin 4a antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an ...read more
Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody [NBP1-31392] - Paraffin-embedded H661 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Pm, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.92 mg/ml

Order Details

alpha Tubulin 4a Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 4a. The exact sequence is proprietary.
Predicted Species
Rat (100%), Primate (100%), Bovine (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TUBA4A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-31392 in the following applications:

Reactivity Notes

Chimpanzee (100%), Xenopus laevis (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.92 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alpha Tubulin 4a Antibody

  • alpha 1 (testis specific)
  • Alpha-tubulin 1
  • FLJ30169
  • H2-ALPHA
  • Testis-specific alpha-tubulin
  • TUBA1
  • Tubulin alpha-1 chain
  • tubulin alpha-4A chain
  • Tubulin H2-alpha
  • tubulin, alpha 1
  • tubulin, alpha 4a

Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-221
Species: Hu, Mu, Rt, Po, Av, Bv, Ch, Eq, Ha, Op, Pm, Sh, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IP
NB500-317
Species: Hu, Bv(-), Ca(-), Ch(-), Mu(-), Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA
NBP1-33527
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24571
Species: Hu, Mu
Applications: WB, Simple Western, IHC-P
NBP2-12933
Species: Hu, Mu, Rt, Po
Applications: WB, PEP-ELISA
NB600-501
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-64792
Species: Hu, Mu, Rt, Rb
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr
NBP2-19784
Species: Hu, Mu, Rt, Ye
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-30682
Species: Hu
Applications: ICC/IF, IHC, IHC-P
248-BD/CF
Species: Hu, Mu, Rt, Ca, Eq
AF2065
Species: Mu
Applications: WB, Simple Western, ICC
NBP1-89105
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-690
Species: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Fu, GP, Ha, Mk, Pa, Pm, Rb, Xp, Ye
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, CyTOF-ready, IF
AF3589
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
NBP2-32972
Species: Hu, Mu, Rt, Ch, Pm, Sh
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB100-1992
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Gt, GP, Ha, Mk, Rb, Sh, Sq, Xp
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
H00009910-M05
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP1-82558
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-31392
Species: Hu, Mu, Rt, Bv, Pm, Sh
Applications: WB, ICC/IF, IHC, IHC-P

Publications for alpha Tubulin 4a Antibody (NBP1-31392)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(2)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for alpha Tubulin 4a Antibody (NBP1-31392) (0)

There are no reviews for alpha Tubulin 4a Antibody (NBP1-31392). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha Tubulin 4a Antibody (NBP1-31392) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional alpha Tubulin 4a Products

Bioinformatics Tool for alpha Tubulin 4a Antibody (NBP1-31392)

Discover related pathways, diseases and genes to alpha Tubulin 4a Antibody (NBP1-31392). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Pathways for alpha Tubulin 4a Antibody (NBP1-31392)

View related products by pathway.

Research Areas for alpha Tubulin 4a Antibody (NBP1-31392)

Find related products by research area.

Blogs on alpha Tubulin 4a.

Tubulin alpha 4A - A ubiquitous tubulin isoform linked to ALS and infertility
Microtubules are a main component of the cytoskeleton and play essential roles in a variety of cellular processes. These highly dynamic tubular structures are assembled from alpha- and beta-tubulin dimers to form a complex structural network of mic...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our alpha Tubulin 4a Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol TUBA4A